Nomura T, Fujita N, Ishihama A
Nucleic Acids Res. 1985 Nov 11;13(21):7647-61. doi: 10.1093/nar/13.21.7647.
Promoter properties were analyzed for the convergently-overlapped E. coli genes coding for the DNA polymerase III epsilon subunit (dnaQ) and the ribonuclease H (rnh). The rates of open complex formation for a single promoter of the rnh gene and two tandem promoters of the dnaQ gene were constant whether they are located on a single DNA fragment or separated into individual fragments. The relative expression levels of these three promoters, as measured using an in vitro mixed transcription system, varied differentially depending on the concentration of RNA polymerase. At low enzyme concentrations, the downstream promoter (P2) of the dnaQ gene was utilized preferentially, but the upstream promoter (P1) was utilized as well when the enzyme concentration was increased. This indicates different physiological roles between the two dnaQ promoters. The level of rnh transcription was as low as that of dnaQ-1 RNA synthesis but the rnh promoter was utilized as well as the dnaQ P2 promoter when it was separated from the dnaQ promoters. This implies a promoter interference between the convergently transcribed genes.
对编码DNA聚合酶IIIε亚基（dnaQ）和核糖核酸酶H（rnh）的大肠杆菌基因的重叠启动子特性进行了分析。rnh基因单个启动子以及dnaQ基因两个串联启动子的开放复合物形成速率是恒定的，无论它们位于单个DNA片段上还是被分隔成单个片段。使用体外混合转录系统测量，这三个启动子的相对表达水平根据RNA聚合酶的浓度而有差异地变化。在低酶浓度下，dnaQ基因的下游启动子（P2）优先被利用，但当酶浓度增加时，上游启动子（P1）也被利用。这表明两个dnaQ启动子之间存在不同的生理作用。rnh转录水平与dnaQ - 1 RNA合成水平一样低，但当rnh启动子与dnaQ启动子分离时，它与dnaQ P2启动子一样被利用。这意味着反向转录基因之间存在启动子干扰。
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