Anderson R L, Hahn G M
Radiat Res. 1985 Jun;102(3):314-23.
The effect of hyperthermia in the 41-50 degrees C range on the Na+,K+-ATPase of CHO cells has been investigated. Three separate activities of the enzyme, namely, ATP hydrolysis, K+ uptake, and binding of the specific inhibitor, ouabain, have been measured independently. The results can be summarized as follows: (1) The ouabain binding capacity of intact cells decreased with increasing temperature and with increasing time at any one temperature. (2) The loss of ouabain-sensitive K+ uptake after treating cells at 45 degrees C was very similar to the loss of ouabain binding capacity. In contrast, the ATP hydrolyzing activity was much more resistant to heat. (3) The ouabain binding capacity was more resistant to hyperthermia at 45 degrees C if the cells were given a prior exposure at 45 degrees C 12 hr earlier. In contrast, the ATP hydrolyzing activity did not display this tolerance to heat.
研究了41 - 50摄氏度范围内的热疗对CHO细胞Na +,K + - ATP酶的影响。该酶的三种独立活性，即ATP水解、K +摄取以及特异性抑制剂哇巴因的结合，已分别进行测定。结果可总结如下：(1)完整细胞的哇巴因结合能力随温度升高以及在任一温度下时间的延长而降低。(2)在45摄氏度处理细胞后，对哇巴因敏感的K +摄取的丧失与哇巴因结合能力的丧失非常相似。相比之下，ATP水解活性对热的耐受性要强得多。(3)如果细胞在12小时前预先暴露于45摄氏度，其哇巴因结合能力在45摄氏度时对热疗的耐受性更强。相比之下，ATP水解活性并未表现出这种耐热性。
