Laboratory of Food Chemistry, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
Laboratory of Food Chemistry, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
Carbohydr Polym. 2018 Oct 1;197:269-276. doi: 10.1016/j.carbpol.2018.06.003. Epub 2018 Jun 4.
The primary plant cell wall is composed of a complex network of pectin, hemicellulose and cellulose. Potential interactions between these polysaccharides were studied for carrot, tomato and strawberry, with a focus on the role of pectin. The Chelating agent Unextractable Solids (ChUS), the residue after water- and EDTA extraction, was ball milled and subsequently water extracted. For tomato and strawberry, pectin and substantial amounts of hemicellulose were solubilised. Anion exchange chromatography (AEC) showed co-elution of pectin and acetylated glucuronoxylan in tomato, representing 18% of solubilised uronic acid and 48% of solubilised xylose by ball milling from ChUS. The existence of a covalently linked pectin-xylan complex was proposed since xylan co-precipitated with pectin under mild alkali conditions. It was proposed that pectin links with xylan through the RG-I region since degradation of HG did not alter AEC elution patterns for RG-I and xylan, suggesting RG-I - xylan interactions.
初生细胞壁由复杂的果胶、半纤维素和纤维素网络组成。研究了这些多糖之间的潜在相互作用，涉及胡萝卜、番茄和草莓，重点是果胶的作用。螯合剂不可提取固体（ChUS）是水和 EDTA 提取后的残渣，经过球磨处理后再用水提取。对于番茄和草莓，果胶和大量半纤维素被溶解。阴离子交换色谱（AEC）显示番茄中果胶和乙酰化葡萄糖醛酸木聚糖共洗脱，占 ChUS 球磨后溶解的糖醛酸的 18%和溶解的木糖的 48%。由于木聚糖在温和的碱性条件下与果胶共沉淀，因此提出了果胶-木聚糖之间存在共价连接的复合物的存在。由于 HG 的降解并没有改变 RG-I 和木聚糖的 AEC 洗脱模式，因此提出果胶通过 RG-I 区域与木聚糖相连，表明 RG-I - 木聚糖相互作用。
