School of Life Science, Anhui Agricultural University, Hefei 230036, China.
Int J Mol Sci. 2018 Aug 2;19(8):2273. doi: 10.3390/ijms19082273.
To investigate the effect of pollination on the fruit quality of 'Dangshan Su' pear, 'Dangshan Su' was fertilized by the pollen of 'Wonhwang' ( Nakai.) (DW) and 'Jingbaili' ( Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantitative analysis of intermediate metabolites of lignin synthesis was undertaken using targeted metabolomics. The untargeted metabolomics analysis was performed via gas chromatography-mass spectrometry (GC-MS). The targeted metabolomics analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. The results showed that the metabolite content was significantly different between DW and DJ. Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs), and the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs. The intermediate metabolites of lignin synthesis were analyzed using the orthogonal partial least squares discriminant analysis (OPLS-DA) model, and the differential metabolites at 47 DAPs were -coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol. The differential significant metabolite at 63 DAPs was -coumaric acid. At 47 DAPs and 63 DAPs, the -coumaric acid level was significantly different, and the -coumaric acid content was positively correlated with lignin synthesis. The pollination pollen affects the quality of 'Dangshan Su' pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the -coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears.
为了研究授粉对“砀山酥”梨果实品质的影响,本研究用“旺红”(Nakai.)(DW)和“京白梨”( Maxim.)(DJ)的花粉对“砀山酥”进行了授粉。通过非靶向代谢组学分析初级代谢物,通过靶向代谢组学分析木质素合成的中间代谢物。非靶向代谢组学分析采用气相色谱-质谱联用(GC-MS)进行,靶向代谢组学分析采用超高效液相色谱-串联质谱联用(UPLC-MS/MS)在多反应监测(MRM)模式下进行。结果表明,DW 和 DJ 之间的代谢物含量存在显著差异。与 DJ 相比,DW 授粉后 47 天(DAPs)的糖和氨基酸含量较高,脂肪酸含量较低,63 DAPs 时 DW 的糖、氨基酸和脂肪酸含量较低。采用正交偏最小二乘判别分析(OPLS-DA)模型分析木质素合成的中间代谢物,在 47 DAPs 时差异代谢物为 -香豆酸、阿魏酸、丁香醛、松柏醇和丁香醇。在 63 DAPs 时的差异显著代谢物为 -香豆酸。在 47 DAPs 和 63 DAPs 时,-香豆酸水平存在显著差异,-香豆酸含量与木质素合成呈正相关。授粉花粉通过调节糖、氨基酸和脂肪酸含量来影响“砀山酥”梨果实的品质;同时,调节木质素合成中间代谢物的水平,特别是 -香豆酸的含量,从而影响木质素合成,最终影响石细胞的含量,提高梨的品质。