Department of Biomechatronic Engineering, National Chiayi University, No. 300 Syuefu Road, Chiayi City 60004, Taiwan.
High Power Opto Incorporation, No. 8, Keyuan 3rd Rd, Xitun Dist., Taichung City 40763, Taiwan.
IET Nanobiotechnol. 2018 Sep;12(6):841-845. doi: 10.1049/iet-nbt.2017.0302.
This study proposes a method for in situ local deoxyribonucleic acid (DNA) replication and detection in a long DNA strand through laser-induced heating and strong avidin-biotin binding. To achieve the target DNA replication, dielectrophoresis was generated to stretch and immobilise DNA strands on both ends of the electrode. Subsequently, local DNA sequences were replicated using thermal cycles generated by laser-induced heating. Replicated double-stranded DNA products were captured in situ on a solid surface and detected using the fluorescence intensity of quantum dots (Qdots). The results revealed that after six laser-induced thermal cycles, the replicated local DNA sequence could be detected by analysing the difference between Qdot fluorescent intensity before and after replication. The proposed method is expected to improve the efficiency of biosample gene sequence analysis.
本研究提出了一种通过激光诱导加热和强链霉亲和素-生物素结合原位进行长链 DNA 局部复制和检测的方法。为了实现目标 DNA 复制，介电泳被用来拉伸和固定电极两端的 DNA 链。随后，通过激光诱导加热产生的热循环复制局部 DNA 序列。复制的双链 DNA 产物在固 体表面原位捕获，并使用量子点（Qdot）的荧光强度进行检测。结果表明，经过六次激光诱导热循环后，通过分析复制前后 Qdot 荧光强度的差异，可以检测到复制的局部 DNA 序列。预计该方法将提高生物样本基因序列分析的效率。
