Gospodarowicz D, Moran J S, Braun D L
J Cell Physiol. 1977 Jun;91(3):377-85. doi: 10.1002/jcp.1040910307.
The effects of Fibroblast Growth Factor (FGF) and Epidermal Growth Factor (EGF) on the proliferation of bovine vascular endothelial cells has been examined. FGF induces the initiation of DNA synthesis and cell proliferation in cloned endothelial cells of fetal and adult origin at concentrations as low as 1 ng/ml and is saturating at 50 ng/ml. EGF had no effect over the same range of concentrations. The mitogenic effect of FGF is blocked by a crude extract of cartilage. Platelet extract is also mitogenic for vascular endothelial cells although to a lesser extent than the purified FGF. In contrast to vascular endothelial cells, both EGF and FGF are mitogenic for vascular smooth muscle cells although EGF is less mitogenic than FGF at 100 ng/ml. The mitogenic effect of EGF and FGF on vascular smooth muscle is not blocked by the addition of a crude extract of cartilage, thus demonstrating the specificity of the chalone like effect of cartilage crude extract for endothelial cells.
研究了成纤维细胞生长因子（FGF）和表皮生长因子（EGF）对牛血管内皮细胞增殖的影响。FGF在低至1 ng/ml的浓度下就能诱导胎儿和成年来源的克隆内皮细胞启动DNA合成和细胞增殖，在50 ng/ml时达到饱和。在相同浓度范围内，EGF没有作用。FGF的促有丝分裂作用被软骨粗提物阻断。血小板提取物对血管内皮细胞也有促有丝分裂作用，尽管程度比纯化的FGF小。与血管内皮细胞不同，EGF和FGF对血管平滑肌细胞都有促有丝分裂作用，尽管在100 ng/ml时EGF的促有丝分裂作用比FGF小。添加软骨粗提物不会阻断EGF和FGF对血管平滑肌的促有丝分裂作用，从而证明了软骨粗提物对内皮细胞的类抑素样作用的特异性。
