抗大鼠肝细胞核 T3 受体抗体的制备。

Production of an antibody against rat liver nuclear T3 receptor.

作者信息

Faure R, Ruel J, Dussault J H

出版信息

Biochem Cell Biol. 1986 Apr;64(4):377-80. doi: 10.1139/o86-052.

Abstract

Rabbits were immunized with rat liver nuclear L-triiodothyronine (T3) receptor purified by preparative sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis using bromoacetyl[125I]T3 as an affinity label. SDS-PAGE confirmed the presence of two receptor forms of the apparent molecular weights 57,000 and 45,000. We describe here a specific antibody, raised against the 57,000 receptor type, which reacts with both receptor forms as assessed by electroimmunoblotting and immunoprecipitation in liquid medium.

摘要

用十二烷基硫酸钠-聚丙烯酰胺（SDS-PAGE）制备性凝胶电泳纯化的大鼠肝细胞核L-三碘甲状腺原氨酸（T3）受体免疫兔子，使用溴乙酰[125I]T3作为亲和标记。SDS-PAGE证实存在两种表观分子量分别为57,000和45,000的受体形式。我们在此描述一种针对57,000受体类型产生的特异性抗体，通过电免疫印迹和液体介质中的免疫沉淀评估，该抗体与两种受体形式都发生反应。