Limas C, Limas C J
Hypertension. 1986 Jul;8(7):566-71. doi: 10.1161/01.hyp.8.7.566.
Development of hypertension in Dahl salt-sensitive rats (DS) is accompanied by reduced renomedullary prostaglandin synthesis, which may be responsible for their lower natriuretic capacity. To examine the changes in renomedullary prostaglandin E2 synthesis, the effects of high (8.0%) and normal (0.6%) NaCl diets were examined in DS and in Dahl salt-resistant rats (DR). In response to an 8.0% NaCl diet, the number of prostaglandin E2 receptors in the renal outer medulla of DR increased (2.97 +/- 0.2 vs 2.18 +/- 0.2 pmol/mg on 0.6% NaCl diet) while no change was noted in their affinities (Kd, 9.5 +/- 0.2 vs 9.4 +/- 0.3 nM). Receptor number and affinity in the renal cortex, inner medulla, and liver of DR were not affected. In contrast, renomedullary receptors of DS had a lower affinity than those of age-matched DR (Kd, 13.9 +/- 0.2 nM on 0.6% NaCl diet and 14.0 +/- 0.3 nM on 8.0% NaCl diet) and did not increase in number after a high salt diet. This apparent inability of DS to modulate prostaglandin receptors may contribute to their susceptibility to salt-induced hypertension.
达尔盐敏感大鼠（DS）高血压的发展伴随着肾髓质前列腺素合成的减少，这可能是其利钠能力较低的原因。为了研究肾髓质前列腺素E2合成的变化，分别在DS大鼠和达尔盐抵抗大鼠（DR）中检测了高盐（8.0%）和正常盐（0.6%）饮食的影响。在8.0% NaCl饮食条件下，DR大鼠肾外髓质中前列腺素E2受体数量增加（0.6% NaCl饮食时为2.18±0.2 pmol/mg，8.0% NaCl饮食时为2.97±0.2 pmol/mg），而其亲和力无变化（解离常数Kd，分别为9.4±0.3 nM和9.5±0.2 nM）。DR大鼠肾皮质、肾内髓质和肝脏中的受体数量和亲和力未受影响。相比之下，DS大鼠肾髓质中的受体亲和力低于年龄匹配的DR大鼠（0.6% NaCl饮食时Kd为13.9±0.2 nM，8.0% NaCl饮食时为14.0±0.3 nM），且高盐饮食后受体数量未增加。DS大鼠这种明显无法调节前列腺素受体的情况可能导致其对盐诱导的高血压易感。
