Spicer L J, Ireland J J
Anal Biochem. 1986 Jul;156(1):25-30. doi: 10.1016/0003-2697(86)90148-x.
Experiments were conducted to compare gonadotropin binding capacity calculated from limited-point saturation analyses to those obtained from Scatchard analyses, and to test the effects of membrane purity and source of gonadotropin receptors on determining the maximum percentage of radioiodinated hormone bound to receptors (maximum bindability). One- to four-point saturation analyses gave results comparable to results by Scatchard analyses when examining relative binding capacities of receptors. Crude testicular homogenates had lower estimates of maximum bindability of 125I-labeled human chorionic gonadotropin than more purified gonadotropin receptor preparations. Under similar preparation techniques, some gonadotropin receptor sources exhibited low maximum bindability.
进行实验以比较通过有限点饱和分析计算出的促性腺激素结合能力与通过Scatchard分析获得的能力，并测试膜纯度和促性腺激素受体来源对确定与受体结合的放射性碘化激素的最大百分比（最大结合能力）的影响。当检查受体的相对结合能力时，一到四点饱和分析得出的结果与Scatchard分析的结果相当。与更纯化的促性腺激素受体制剂相比，粗制睾丸匀浆对125I标记的人绒毛膜促性腺激素的最大结合能力估计值较低。在相似的制备技术下，一些促性腺激素受体来源表现出较低的最大结合能力。
