建立并优化顶空固相微萃取-气相色谱-质谱联用方法以量化波特酒中的挥发性羰基化合物。
Development and optimization of a HS-SPME-GC-MS methodology to quantify volatile carbonyl compounds in Port wines.
机构信息
UCIBIO@REQUIMTE/Laboratório de Toxicologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal; LQAV/REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
UCIBIO@REQUIMTE/Laboratório de Toxicologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
出版信息
Food Chem. 2019 Jan 1;270:518-526. doi: 10.1016/j.foodchem.2018.07.093. Epub 2018 Jul 17.
A method based on headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-triple quadrupole/mass spectrometry detection (GC-TQ/MS) with a prior derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) was developed to quantify carbonyl compounds in different categories of Port wines. Optimal extraction conditions were obtained incubating 2 ml of wine with 2.3 g/l of PFBHA for 10 min and extracted during 20 min at 32 °C. The method was validated for 38 carbonyl compounds (alkanals, alkenals, Strecker aldehydes, dialdehydes, ketones and furan aldehydes) with regard to linearity, repeatability, inter and intra-day precision and accuracy, showing that the method is suitable for the determination of carbonyl compounds in wines. Tawny wines with 'indication of age' (10-40 years old) presented the highest levels of some carbonyl compounds, such as propanal, pentanal, hexanal, Strecker aldehydes, diacetyl, methyl glyoxal, 3-pentanone and 2-furfural, whereas Ruby wines were characterized by the highest amounts of some unidentified compounds.
建立了一种基于顶空固相微萃取（HS-SPME）结合气相色谱-三重四极杆/质谱检测（GC-TQ/MS）的方法，该方法在衍生化步骤中使用 O-（2,3,4,5,6-五氟苄基）羟胺盐酸盐（PFBHA），用于定量不同类别波特酒中的羰基化合物。优化的提取条件为：用 2.3 g/L 的 PFBHA 孵育 2 ml 葡萄酒 10 min，然后在 32°C 下提取 20 min。该方法对 38 种羰基化合物（烷醛、烯醛、Strecker 醛、二醛、酮和糠醛）进行了线性、重复性、日内和日间精密度和准确度的验证，表明该方法适用于葡萄酒中羰基化合物的测定。具有“陈酿年份”（10-40 年）的茶色波特酒中某些羰基化合物（如丙醛、戊醛、己醛、Strecker 醛、双乙酰、甲基乙二醛、3-戊酮和 2-糠醛）的含量最高，而红宝石波特酒的某些未鉴定化合物的含量最高。
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