School of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, Liaoning, China.
School of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, Liaoning, China.
Mar Environ Res. 2018 Oct;141:138-147. doi: 10.1016/j.marenvres.2018.08.010. Epub 2018 Aug 17.
Stranding of oil onto a coastline after an oil spill threatens the health of marine benthic organisms. Here, the transgenerational effects of exposure to stranded heavy fuel oil (HFO) on the sea urchin Strongylocentrotus intermedius were assessed. The column containing gravel coated with HFO was prepared in the laboratory to simulate HFO-contaminated gravel shorelines. Adult sea urchins were exposed for 21 days to either a HFO-oiled gravel column at the oil loading of 3000 μg oil/g gravel or a non-HFO-oiled gravel column (as the control treatment) and then offspring were either exposed to HFO or ambient seawater conditions. The sublethal exposure to HFO for 21 days induced polycyclic aromatic hydrocarbons (PAHs) accumulation in gonads, accompanied by increased levels of oxidative lipid, protein and DNA damage and a reduction in total antioxidant capacity. Analysis of gametes indicated that both maternal and paternal exposure could result in the transfer of PAHs and DNA damage to their offspring. Parental (maternal, paternal or both) exposure to HFO caused increases in malformation rates of offspring compared to those from control parents under ambient seawater condition. Continued HFO exposure in the offspring resulted in further increased malformation rates compared with those reared in ambient seawater, as well as oxidative lipid, protein and DNA damage. Furthermore, mother exposure history reduced the total antioxidant capacity of larvae to response to continued HFO exposure. Overall, the results suggest an increased sensitivity to toxic effects of HFO in larvae from exposed both parents compared with those from control parents in S. intermedius, which may consequently affect the recruitment and population maintenance.
溢油事故发生后，油污在海岸线上搁浅，这威胁到海洋底栖生物的健康。在这里，研究了暴露于搁浅重质燃料油（HFO）对中间石斑鱼（Strongylocentrotus intermedius）的跨代影响。在实验室中制备了涂有 HFO 的砾石柱，以模拟受 HFO 污染的砾石海岸线。成年海胆暴露于 HFO 油砾石柱或非 HFO 油砾石柱（对照处理）中 21 天，然后将后代暴露于 HFO 或环境海水条件下。亚致死浓度的 HFO 暴露 21 天导致性腺中多环芳烃（PAHs）积累，同时氧化脂质、蛋白质和 DNA 损伤水平增加，总抗氧化能力降低。配子分析表明，母体和父体暴露都可能导致 PAHs 和 DNA 损伤传递给后代。与在环境海水条件下来自对照亲代的后代相比，亲代（母体、父体或两者）暴露于 HFO 会导致后代畸形率增加。与在环境海水中饲养的后代相比，后代持续暴露于 HFO 会导致进一步增加畸形率，以及氧化脂质、蛋白质和 DNA 损伤。此外，母亲暴露史降低了幼虫对持续 HFO 暴露的总抗氧化能力。总体而言，研究结果表明，与来自对照亲代的后代相比，暴露于 HFO 的双亲后代对 HFO 的毒性效应更为敏感，这可能会影响中间石斑鱼的补充和种群维持。
