人类15号染色体上三个多态性DNA片段的区域定位。

Regional assignments of three polymorphic DNA segments on human chromosome 15.

作者信息

Brissenden J E, Page D C, de Martinville B, Trowsdale J, Botstein D, Francke U

出版信息

Genet Epidemiol. 1986;3(4):231-9. doi: 10.1002/gepi.1370030404.

Abstract

Hybridization of probe pDP151 (locus D15S2) to genomic human DNAs digested with EcoRI revealed allelic restriction fragments 9 and 11 kilobase-pairs (kb) in length. Hybridization of pDP151 to EcoRI-digested DNAs from 21 Chinese hamster X human hybrid cell clones containing different subsets of human chromosomes demonstrated cosegregation of the 9 and 11 kb EcoRI fragments with human chromosome 15. D15S2 and two other polymorphic loci previously mapped to chromosome 15--D15S1 and D15S6--were localized to specific regions on human chromosome 15. Eight Chinese hamster X human somatic cell hybrid clones derived from a human donor heterozygous for a balanced translocation between chromosomes 15 and 22 [t(15;22)(q14;q13.3); Oliver et al, Cytogenet Cell Genet 22:503-510, 1978] were studied. After digestion of human and hybrid DNAs with HindIII and Southern blotting, pDP151 (D15S2) and pMS1-14 (D15S1) hybridized to fragments of 4 and 4.5 kb, respectively. Further, pMS1-14 (D15S1) and p9-1a (D15S6) hybridized to EcoRI fragments of 3.5 and 3.2 kb. All fragments cosegregated with the der(22) derivative chromosome containing region 15q14----15qter. In situ hybridization of these probes to normal human chromosomes mapped the corresponding loci with greater precision: D15S1 to 15q15----15q21, D15S2 to 15q15----15q22, and D15S6 to 15q22----15q24.

摘要

用EcoRI消化的人基因组DNA与探针pDP151（基因座D15S2）杂交，显示出长度为9和11千碱基对（kb）的等位基因限制性片段。pDP151与来自21个含有不同人类染色体亚群的中国仓鼠×人类杂交细胞克隆的EcoRI消化DNA杂交，证明9 kb和11 kb的EcoRI片段与人类15号染色体共分离。D15S2以及先前定位到15号染色体的其他两个多态性基因座——D15S1和D15S6——被定位到人类15号染色体的特定区域。研究了来自一名人类供体的八个中国仓鼠×人类体细胞杂交克隆，该供体在15号和22号染色体之间存在平衡易位[t(15;22)(q14;q13.3); Oliver等人，《细胞遗传学与细胞遗传学》22:503 - 510, 1978]。用HindIII消化人类和杂交DNA并进行Southern印迹分析后，pDP151（D15S2）和pMS1 - 14（D15S1）分别与4 kb和4.5 kb的片段杂交。此外，pMS1 - 14（D15S1）和p9 - 1a（D15S6）与3.5 kb和3.2 kb的EcoRI片段杂交。所有片段都与含有15q14----15qter区域的der(22)衍生染色体共分离。这些探针与正常人类染色体的原位杂交更精确地定位了相应基因座：D15S1位于15q15----15q21，D15S2位于15q15----15q22，D15S6位于15q22----15q24。