Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai 200072, PR China.
Institute of Bone Tumor Affiliated to Tongji University, School of Medicine, Shanghai 200072, PR China.
Epigenomics. 2018 Oct;10(10):1327-1346. doi: 10.2217/epi-2018-0023. Epub 2018 Sep 7.
To identify circular RNAs (circRNAs) related to osteosarcoma (OS) chemoresistance.
MATERIALS & METHODS: CircRNA expression profile was performed in three paired human chemoresistant and chemosensitive OS cell lines by next-generation sequencing. Quantitative real-time-PCR (qRT-PCR) was used to confirm next-generation sequencing data. Bioinformatics analysis was conducted to predict their functions.
Eighty circRNAs were dysregulated in the chemoresistant OS cells compared with the control, after validated by qRT-PCR. Bioinformatics analysis showed that some pathways related to drug metabolism were significantly enriched. Additionally, hsa_circ_0004674 was distinctly increased in OS chemoresistant cells and tissues, related to poor prognosis. CircRNA-miRNA-mRNA pathways related to hsa_circ_0004674 were constructed by TargetScan and miRanda.
CircRNAs may play a role in OS chemoresistance and hsa_circ_0004674 might be a candidate target.
鉴定与骨肉瘤（OS）化疗耐药相关的环状 RNA（circRNAs）。
通过下一代测序对三对人源化疗耐药和化疗敏感 OS 细胞系中的 circRNA 表达谱进行分析。采用实时定量 PCR（qRT-PCR）对下一代测序数据进行验证。通过生物信息学分析预测其功能。
通过 qRT-PCR 验证，与对照相比，耐药 OS 细胞中 80 个 circRNAs 发生了失调。生物信息学分析显示，一些与药物代谢相关的通路显著富集。此外，hsa_circ_0004674 在 OS 耐药细胞和组织中明显增加，与不良预后相关。通过 TargetScan 和 miRanda 构建了与 hsa_circ_0004674 相关的 circRNA-miRNA-mRNA 通路。
circRNAs 可能在 OS 化疗耐药中发挥作用，hsa_circ_0004674 可能是一个候选靶点。
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