猪CD83是一种糖基化二聚体蛋白，天然以膜结合形式和可溶性形式存在。
Porcine CD83 is a glycosylated dimeric protein existing naturally in membrane-bound and soluble forms.
作者信息
Huo Shanshan, Zhang Jianlou, Wu Fengyang, Zuo Yuzhu, Cui Dan, Li Xiujin, Zhong Zhenyu, Zhong Fei
机构信息
Laboratory of Molecular Virology and Immunology, College of Animal Science and Technology/College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei, 071000, China.
Laboratory of Molecular Virology and Immunology, College of Animal Science and Technology/College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, 071000, China.
出版信息
Dev Comp Immunol. 2019 Jan;90:60-69. doi: 10.1016/j.dci.2018.09.002. Epub 2018 Sep 5.
Human and mouse CD83 have been well characteized, however, the other mammalian CD83 genes have not been cloned and characterized. In this study, the porcine CD83 (pCD83) was cloned, expressed and characterized, and showed that the pCD83 gene has 81% and 74% homologies with humans and mice, respectively, which was identified to be glycosylated when expressed in eukaryotic cells, existing naturally in two forms: membrance-bound CD83 (mCD83) and soluble CD83 (sCD83), the latter was identified to be generated mainly from mCD83 by proteolytic shedding. The pCD83 was a dimmer mediated by intermolecular disulfide bond formed by the fifth cysteine in the exrtracellular domain. Functionally, the recombinant porcine sCD83 was preliminarily tested to have the ability to inhibit DC-mediated T cell activition. This study provided necessary fundation for further investigation on pCD83 functions.
人类和小鼠的CD83已得到充分表征，然而，其他哺乳动物的CD83基因尚未被克隆和表征。在本研究中，猪CD83（pCD83）被克隆、表达和表征，结果表明pCD83基因与人类和小鼠的同源性分别为81%和74%，在真核细胞中表达时被鉴定为糖基化，天然以两种形式存在：膜结合型CD83（mCD83）和可溶性CD83（sCD83），后者主要通过蛋白水解脱落从mCD83产生。pCD83是一种二聚体，由细胞外结构域中第五个半胱氨酸形成的分子间二硫键介导。在功能上，重组猪sCD83经初步测试具有抑制树突状细胞介导的T细胞活化的能力。本研究为进一步研究pCD83的功能提供了必要的基础。
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