Leviel V, Charriere B, Fayada C, Guibert B
Brain Res. 1986 Sep 3;381(2):205-14. doi: 10.1016/0006-8993(86)90069-7.
In order to study the mechanisms regulating the dopaminergic nigroamygdaloid cells, the release of dopamine was observed in the central nucleus of the amygdaloid complex. Halothane anesthetized rats were implanted, according to the experiment, with one or two push-pull cannulae in the central nuclei of the amygdala (ACE), the substantia nigra (SN) and/or the caudate nucleus (CN). Canulae were supplied with artificial cerebrospinal fluid (CSF) containing tritiated tyrosine, and labeled dopamine [3H]DA was evaluated in successive superfusate fractions. Electrical stimulation of the medial forebrain bundle with an implanted bipolar electrode induced an increase of the [3H]DA release in the ipsi- and contralateral ACE. Electrical stimulation of the SN produced only a very delayed effect in the ipsilateral ACE but an immediate and large increase of [3H]DA release in the contralateral structure. Superfusion of unlabeled DA and alpha-methyl-p-tyrosine in the SN remained ineffective on the [3H]DA release in the ipsilateral ACE. In this structure the release of [3H]DA was, however, decreased by nigral superfusion with gamma-amino-butyric acid (GABA). D-(+)-Amphetamine (1 microM), when superfused in the CN, induced a large enhancement of the [3H]DA release in the ipsilateral ACE simultaneously with the local increase of [3H]DA release. The results presented here are in agreement with the previous studies concerning the anatomical organization of the dopaminergic nigroamygdaloid pathway. The DA cell bodies located in the SN appear insensitive to a local action of DA, perhaps due to a lack of autoreceptors. They are, however, powerfully inhibited by GABA and the relation observed between the [3H]DA release in the CN and ACE support the hypothesis that the SN can act as a relay between the extrapyramidal and limbic systems.
为了研究调节多巴胺能黑质杏仁核细胞的机制，在杏仁复合体中央核中观察了多巴胺的释放。根据实验，将氟烷麻醉的大鼠在杏仁核中央核（ACE）、黑质（SN）和/或尾状核（CN）植入一根或两根推挽式套管。套管中注入含有氚化酪氨酸的人工脑脊液（CSF），并在连续的灌流液部分中评估标记的多巴胺[3H]DA。用植入的双极电极电刺激内侧前脑束可诱导同侧和对侧ACE中[3H]DA释放增加。电刺激SN对同侧ACE中[3H]DA释放仅产生非常延迟的影响，但对侧结构中[3H]DA释放立即大幅增加。在SN中灌注未标记的DA和α-甲基-p-酪氨酸对同侧ACE中[3H]DA释放仍然无效。然而，在该结构中，用γ-氨基丁酸（GABA）灌注黑质可使[3H]DA释放减少。D-（+）-苯丙胺（1 microM）在CN中灌注时，可诱导同侧ACE中[3H]DA释放大幅增强，同时局部[3H]DA释放增加。此处呈现的结果与先前关于多巴胺能黑质杏仁核通路解剖组织的研究一致。位于SN的DA细胞体似乎对DA的局部作用不敏感，这可能是由于缺乏自身受体。然而，它们受到GABA的强烈抑制，并且在CN和ACE中观察到的[3H]DA释放之间的关系支持了SN可作为锥体外系和边缘系统之间中继的假说。
