弗鲁他林和阿霉素对体外培养的山羊次级卵泡生长、超微结构及基因表达的影响。
Effects of frutalin and doxorubicin on growth, ultrastructure and gene expression in goat secondary follicles cultured in vitro.
作者信息
Soares-Costa M A A, Costa J J N, Silva A W B, Cunha E V, Paulino L R F M, Silva B R, Silva A L C, van den Hurk R, Silva J R V
机构信息
Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceará, Sobral, Ceará, Brazil.
Molecular Biotechnology Laboratory (LabBMol), Federal University of Ceará, Fortaleza, Ceará, Brazil.
出版信息
Res Vet Sci. 2018 Oct;120:33-40. doi: 10.1016/j.rvsc.2018.08.006. Epub 2018 Aug 30.
This study evaluated the effects of frutalin (0.6, 6.0 or 60.0 μg/mL) and doxorubicin (0.3 μg/mL) on survival, growth and ultrastructure of in-vitro cultured goat secondary follicles. The effects of these substances on the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 were also investigated. Results showed that, after 6 days of culture, frutalin or doxorubicin reduced the percentage of normal follicles (P < 0.05), but doxorubicin had higher toxicity than frutalin. Except for follicles cultured with 0.6 μg/mL frutalin, follicular growth rate was reduced after culture with doxorubicin or frutalin (P < 0.05). The presence doxorubicin or 60.0 μg/mL frutalin increased the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 (P < 0.05). Higher mRNA levels for Casp3, Casp6 and Bax were found in follicles cultured with doxorubicin, but higher levels of Bcl2 mRNA were found in follicles cultured with frutalin (P < 0.05). In conclusion, frutalin has lower toxic effects than doxorubicin on secondary follicles cultured in vitro.
本研究评估了弗鲁他林（0.6、6.0或60.0μg/mL）和阿霉素（0.3μg/mL）对体外培养的山羊次级卵泡存活、生长及超微结构的影响。还研究了这些物质对Casp3、Casp6、Bax和Bcl2 mRNA水平的影响。结果显示，培养6天后，弗鲁他林或阿霉素降低了正常卵泡的百分比（P<0.05），但阿霉素的毒性高于弗鲁他林。除用0.6μg/mL弗鲁他林培养的卵泡外，用阿霉素或弗鲁他林培养后卵泡生长速率降低（P<0.05）。阿霉素或60.0μg/mL弗鲁他林的存在增加了Casp3、Casp6、Bax和Bcl2的mRNA水平（P<0.05）。在用阿霉素培养的卵泡中发现Casp3、Casp6和Bax的mRNA水平较高，但在用弗鲁他林培养的卵泡中发现Bcl2 mRNA水平较高（P<0.05）。总之，弗鲁他林对体外培养的次级卵泡的毒性作用低于阿霉素。
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