Ross A H, Pleasure D, Sonnenfeld K, Atkinson B, Kreider B, Jackson D M, Taff I, Scarpini E, Lisak R P, Koprowski H
Cancer Res. 1986 Nov;46(11):5887-92.
The cell surface antigen distribution on traumatic neuroma Schwann cells and neurofibroma Schwann-like cells was characterized using monoclonal antibodies that define melanoma-associated antigens. Immunofluorescence staining of cultured cells, immunoprecipitation of radioiodinated antigens from cells placed in short-term cultures, and immunoperoxidase staining of frozen tissue sections revealed most of the melanoma-associated antigens tested on traumatic neuroma and neurofibroma Schwann cells and on fetal and adult femoral nerve. The cross-reactivity of the antibodies with neural cells may reflect the common neural crest embryological origin of Schwann cells and melanocytes. Cell sorter analysis of neurofibroma cells using a monoclonal antibody directed against the melanoma nerve growth factor receptor resulted in cell cultures highly enriched for Schwann-like cells which may bear the genetic defect responsible for neurofibromatosis. The antigen detected by this monoclonal antibody is the neurofibroma nerve growth factor receptor and the antibody was a potent inhibitor of nerve growth factor binding to neurofibroma cells.
利用可识别黑色素瘤相关抗原的单克隆抗体，对创伤性神经瘤雪旺细胞和神经纤维瘤雪旺样细胞的细胞表面抗原分布进行了表征。对培养细胞进行免疫荧光染色、对短期培养细胞中的放射性碘化抗原进行免疫沉淀，以及对冷冻组织切片进行免疫过氧化物酶染色，结果显示，在创伤性神经瘤和神经纤维瘤雪旺细胞以及胎儿和成人股神经上测试的大多数黑色素瘤相关抗原均呈阳性。抗体与神经细胞的交叉反应可能反映了雪旺细胞和黑素细胞共同的神经嵴胚胎起源。使用针对黑色素瘤神经生长因子受体的单克隆抗体对神经纤维瘤细胞进行细胞分选分析，得到了高度富集雪旺样细胞的细胞培养物，这些细胞可能携带导致神经纤维瘤病的基因缺陷。该单克隆抗体检测到的抗原是神经纤维瘤神经生长因子受体，并且该抗体是神经生长因子与神经纤维瘤细胞结合的有效抑制剂。
