Bristow D R, Bowery N G, Woodruff G N
Eur J Pharmacol. 1986 Jul 31;126(3):303-7. doi: 10.1016/0014-2999(86)90062-2.
Receptor autoradiography has been employed to determine the distribution of strychnine-insensitive glycine binding sites in rat brain using [3H]glycine as a ligand. The location was significantly different from and more widespread than glycine sensitive [3H]strychnine binding sites. Highest binding densities were observed in hippocampus, cortex, subiculum and amygdala followed by striatum, cerebellum and olfactory areas. Characterisation of the binding indicated that it was saturable, of high affinity, stereoselective and displaced by structurally related amino acids. The results support the existence of two glycine receptor subtypes: strychnine-sensitive and strychnine-insensitive.
利用[3H]甘氨酸作为配体，采用受体放射自显影术来测定大鼠脑中对士的宁不敏感的甘氨酸结合位点的分布。其分布位置与对甘氨酸敏感的[3H]士的宁结合位点显著不同，且分布更广泛。在海马体、皮质、下托和杏仁核中观察到最高的结合密度，其次是纹状体、小脑和嗅觉区域。结合特性表明其具有可饱和性、高亲和力、立体选择性且可被结构相关的氨基酸取代。这些结果支持存在两种甘氨酸受体亚型：对士的宁敏感的和对士的宁不敏感的。
