Hori M, Gemba M
J Pharmacobiodyn. 1986 May;9(5):510-2. doi: 10.1248/bpb1978.9.510.
We studied the effect of cyclic guanosine 3',5'-monophosphate (cyclic GMP) on p-aminohippurate (PAH) uptake by basolateral membrane vesicles isolated from rat kidney cortex. Cyclic GMP inhibited PAH uptake dose-dependently. Dibutyryl cyclic GMP inhibited the uptake of PAH to the same extent. However, cyclic adenosine 3',5'-monophosphate, cyclic cytidine 3',5'-monophosphate and guanosine monophosphate had no effect on PAH uptake by membrane vesicles. Therefore, the inhibition of PAH uptake was specific to cyclic GMP and not common to nucleotides. In the presence of probenecid, an inhibitor of PAH transport, cyclic GMP did not affect PAH uptake. Thus, cyclic GMP had an inhibitory effect on probenecid-sensitive PAH transport. Inhibition by cyclic GMP of PAH uptake by basolateral membrane vesicles as described this study may contribute to the decrease in PAH accumulation in kidney cortical slices caused by the cyclic nucleotide which we previously reported.
我们研究了环磷酸鸟苷（cGMP）对从大鼠肾皮质分离的基底外侧膜囊泡摄取对氨基马尿酸（PAH）的影响。cGMP剂量依赖性地抑制PAH摄取。二丁酰环磷鸟苷对PAH摄取的抑制程度相同。然而，环磷酸腺苷、环磷酸胞苷和鸟苷单磷酸对膜囊泡摄取PAH没有影响。因此，PAH摄取的抑制作用对cGMP具有特异性，而非核苷酸所共有。在PAH转运抑制剂丙磺舒存在的情况下，cGMP不影响PAH摄取。因此，cGMP对丙磺舒敏感的PAH转运具有抑制作用。本研究中所述的cGMP对基底外侧膜囊泡摄取PAH的抑制作用可能有助于解释我们之前报道的环核苷酸导致肾皮质切片中PAH蓄积减少的现象。
