Koren H S, Hodes R J
Eur J Immunol. 1977 Jun;7(6):394-400. doi: 10.1002/eji.1830070613.
In agreement with previous reports, the primary in vitro response to alloantigens has been shown to be dependent on the presence of macrophages (Mphs). Splenocytes extensively depleted of adherent phagocytic cells did not generate cytotoxic T lymphocytes, and this activity could be completely restored by small numbers of adherent peritoneal cells (accessory cells). Either P388D1 (Mph-like tumor), P388 ("null" tumor) or P815 (mastocytoma) tumor cells, or 2-mercaptoethanol, could completely replace the accessory function normally mediated by accessory cells. These tumor cells did not non-specifically "enhance" the cytotoxic activity generated with normal nondepleted spleen cells. The restored cultures maintained killing specificity to H-2 targets which was mediated by effector T cells as shown by sensitivity to anti-theta and complement. Therefore, Mphs seem not to be the sole cells capable of mediating an accessory function in a primary response to alloantigens in vitro.
与先前的报道一致，体外对同种异体抗原的主要反应已被证明依赖于巨噬细胞（Mphs）的存在。大量去除黏附吞噬细胞的脾细胞不会产生细胞毒性T淋巴细胞，而少量黏附的腹腔细胞（辅助细胞）可完全恢复这种活性。P388D1（类巨噬细胞瘤）、P388（“无”肿瘤）或P815（肥大细胞瘤）肿瘤细胞，或2-巯基乙醇，均可完全替代辅助细胞通常介导的辅助功能。这些肿瘤细胞不会非特异性地“增强”正常未去除脾细胞产生的细胞毒性活性。恢复后的培养物对H-2靶标的杀伤特异性得以维持，如对抗θ和补体的敏感性所示，这种特异性由效应T细胞介导。因此，巨噬细胞似乎不是体外对同种异体抗原初次反应中唯一能够介导辅助功能的细胞。
