[miR-98-5p promotes apoptosis and inhibits migration by reducing the level of STAT3 in A549 cells].

Objective To explore the effects of microRNA-98-5p (miR-98-5p) on the growth of lung cancer A549 cells and its mechanism. Methods The expression of miR-98-5p in the normal human bronchial epithelial (HBE) cell line and NSCLC cell lines (A549, H1299 and H460) was detected using real-time quantitative PCR. The effects of miR-98-5p mimic and miR-98-5p inhibitor (anti-miR-98-5p) on A549 cell proliferation were detected with CCK-8 assay. The influence of miR-98-5p on the apoptosis of A549 cells was tested by flow cytometry. The activities of caspase-3 and caspase-9 were determined by commercial kits. The effects of miR-98-5p on the invasion and migration of A549 cells were detected with Transwell assay. The effects of miR-98-5p on the expression of STAT3 in A549 cells were verified using real-time quantitative PCR and Western blot analysis. Results Compared with HBE cells, the expression of miR-98-5p in A549, H1299, and H460 cells decreased markedly. The miR-98-5p mimic reduced cell proliferation, promoted cell apoptosis, and inhibited the migration of A549 cells. Meanwhile, miR-98-5p mimic significantly reduced the expression of STAT3 in A549 cells. Conclusion The miR-98-5p promotes apoptosis, inhibits migration of A549 cells by down-regulating STAT3 expression.