Sun Lijin, Zhang Zhongbao, Zeng Bo, Chen Yongmei, Li Hao, Zhou Hongmei, Zhang Zhi, Li Zaixin
Department of Biological Engineering, Sichuan University of Science & Engineering, Zigong 643000, China.
School of Chemical Engineering, Sichuan University of Science & Engineering, Zigong 643000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Jun;34(6):555-560.
Objective To prepare a specific multivalent immunoglobulin of yolk (IgY) against multi-virulence factors of Helicobacter pylori (Hp). Methods The purified recombinant BabA2, UerB and FlaA of Hp were mixed equally with adjuvant, and then used to immunize egg-laying hens to produce specific multivalent IgY. Next, the biological characteristics of purified IgY were analyzed by SDS-PAGE, Western blot and ELISA. To analyze the inhibition effects of multivalent IgY on the growth of Hp in vitro, Hp was co-cultured with different final concentration of IgY (1, 5, 10) mg/mL in the presence or absence of antibiotics. Results After immunization, the titer of egg yolk IgY reached up to 1:150 000. Furthermore, the bacteriostasis assay showed that the multivalent IgY had a significant inhibitory effect on the growth of Hp at 5 mg/mL, and the combination with amoxicillin could further inhibited the growth of Hp. Conclusion The specific multivalent IgY against Hp is successfully prepared.
目的 制备针对幽门螺杆菌（Hp）多种毒力因子的特异性多价卵黄免疫球蛋白（IgY）。方法 将纯化的Hp重组BabA2、UerB和FlaA与佐剂等比例混合，然后用于免疫产蛋母鸡以产生特异性多价IgY。接下来，通过SDS-PAGE、Western印迹和ELISA分析纯化IgY的生物学特性。为了分析多价IgY对Hp体外生长的抑制作用，在有或没有抗生素的情况下，将Hp与不同终浓度（1、5、10）mg/mL的IgY共培养。结果 免疫后，卵黄IgY效价高达1:150 000。此外，抑菌试验表明，多价IgY在5 mg/mL时对Hp的生长具有显著抑制作用，与阿莫西林联合使用可进一步抑制Hp的生长。结论 成功制备了针对Hp的特异性多价IgY。
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