Ohashi Yoshinori, Nakase Junsuke, Shimozaki Kengo, Torigoe Kojun, Tsuchiya Hiroyuki
Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.
Department of Rehabilitation, Fukui Health Science University Faculty of Health Science, Fukui, Japan.
J Exp Orthop. 2018 Sep 21;5(1):37. doi: 10.1186/s40634-018-0152-6.
Using the film model method, the process whereby a substance called tendon gel is secreted from transected tendon ends and changed into a tendon after application of a traction force is known. The objective of this study was to investigate the association between mechanical properties in the early stages of tendon regeneration and time by using the film model method.
Adult male ddY mice, closed colony mice established and maintained in Japan, were prepared for each experimental group. The study animals were 30 mice and were divided into three groups of 10 mice each. Ten specimens of tendon gel secreted from the transected tendon ends were collected on days 10, 15, and 20 postoperatively. While a traction force of 0.00245 N was applied to these specimens, the process of tendon gel changing into a tendon was video recorded for 24 h, and the length of extension was measured over time. Regenerated tendons were stained with hematoxylin and eosin for histological examination. Healing site was studied histologically according to the our maturity score with reference to the Bonar's scale.
The day 10 specimens gradually stretched for 12 h after the start of pulling and transformed into tendons. In contrast, the day 15 and 20 specimens stretched immediately after the start of pulling and transformed into tendons. The day 10 specimens stretched significantly more than the day 15 and 20 specimens (mechanical strain; 0.43 ± 0.26%, 0.03 ± 0.02%, and 0.03 ± 0.01%, respectively)Statistically significant differences were observed in the day 10 specimens than in the day 15 and 20 specimens. (P < 0.017). Using our maturity scores, the day 15 and 20 specimens were more mature than the day 10 specimens. (1.6 ± 0.68, 3.9 ± 0.54, and 4.8 ± 0.64, respectively) Statistically significant differences were observed in the day 10 specimens than in the day 15 and 20 specimens (P < 0.017).
Tendon gel physiologically and histologically matures on or after day 15 and becomes stronger dynamically in mechanical strength after day 15 than after day 10.
采用薄膜模型法，已知一种名为肌腱凝胶的物质从横断的肌腱末端分泌出来，并在施加牵引力后转变为肌腱的过程。本研究的目的是通过薄膜模型法研究肌腱再生早期阶段的力学性能与时间之间的关系。
为每个实验组准备成年雄性ddY小鼠，这是在日本建立并维持的封闭群体小鼠。研究动物为30只小鼠，分为三组，每组10只。在术后第10天、15天和20天收集从横断肌腱末端分泌的10个肌腱凝胶标本。在对这些标本施加0.00245 N的牵引力时，将肌腱凝胶转变为肌腱的过程进行24小时视频记录，并随时间测量伸展长度。对再生肌腱进行苏木精和伊红染色以进行组织学检查。根据我们的成熟度评分并参考博纳尔量表对愈合部位进行组织学研究。
第10天的标本在开始拉伸后逐渐伸展12小时并转变为肌腱。相比之下，第15天和20天的标本在开始拉伸后立即伸展并转变为肌腱。第10天的标本伸展程度明显大于第15天和20天的标本（机械应变分别为0.43±0.26%、0.03±0.02%和0.03±0.01%）。第10天的标本与第15天和20天的标本相比，观察到统计学上的显著差异（P<0.017）。使用我们的成熟度评分，第15天和20天的标本比第10天的标本更成熟（分别为1.6±0.68、3.9±0.54和4.8±0.64）。第10天的标本与第15天和20天的标本相比，观察到统计学上的显著差异（P<0.017）。
肌腱凝胶在生理和组织学上在第15天或之后成熟，并且在第15天后其力学强度在动态上比第10天后更强。
