GRASP55在葡萄糖剥夺条件下促进自噬体成熟。
GRASP55 facilitates autophagosome maturation under glucose deprivation.
作者信息
Zhang Xiaoyan, Wang Yanzhuang
机构信息
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Department of Neurology, University of Michigan School of Medicine, Ann Arbor, MI, USA.
出版信息
Mol Cell Oncol. 2018 Aug 6;5(4):e1494948. doi: 10.1080/23723556.2018.1494948. eCollection 2018.
Abstract
Under growth condition, the Golgi reassembly-stacking protein of 55 kDa (GRASP55)/GORASP2 serves as the "glue" to hold adjacent Golgi cisternae into stacks by forming -oligomers. Upon glucose starvation, GRASP55 is de-O-GlcNAcylated and functions as a membrane tether to facilitate autophagosome-lysosome fusion through the interactions with LC3-II and LAMP2.
摘要
在生长条件下，55 kDa的高尔基体重新组装-堆叠蛋白（GRASP55）/GORASP2通过形成寡聚体充当“胶水”，将相邻的高尔基体潴泡维系成堆叠结构。在葡萄糖饥饿时，GRASP55发生O-连接N-乙酰葡糖胺去修饰，并作为膜系链蛋白发挥作用，通过与LC3-II和LAMP2相互作用促进自噬体-溶酶体融合。
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本文引用的文献
1
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Dev Cell. 2018 Apr 23;45(2):245-261.e6. doi: 10.1016/j.devcel.2018.03.023.
2
Protein O-GlcNAcylation: emerging mechanisms and functions.
Nat Rev Mol Cell Biol. 2017 Jul;18(7):452-465. doi: 10.1038/nrm.2017.22. Epub 2017 May 10.
3
The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Mol Biol Cell. 2014 Apr;25(8):1327-37. doi: 10.1091/mbc.E13-08-0447. Epub 2014 Feb 19.
4
The machinery of macroautophagy.
Cell Res. 2014 Jan;24(1):24-41. doi: 10.1038/cr.2013.168. Epub 2013 Dec 24.
5
Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65.
Nat Commun. 2013;4:1659. doi: 10.1038/ncomms2669.
6
Secretory versus degradative autophagy: unconventional secretion of inflammatory mediators.
J Innate Immun. 2013;5(5):471-9. doi: 10.1159/000346707. Epub 2013 Feb 22.
7
The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001.
8
GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.
J Cell Biol. 2010 Jan 25;188(2):237-51. doi: 10.1083/jcb.200907132. Epub 2010 Jan 18.
9
Role for Rab7 in maturation of late autophagic vacuoles.
J Cell Sci. 2004 Sep 15;117(Pt 20):4837-48. doi: 10.1242/jcs.01370. Epub 2004 Aug 31.
10
Rab7 is required for the normal progression of the autophagic pathway in mammalian cells.
J Cell Sci. 2004 Jun 1;117(Pt 13):2687-97. doi: 10.1242/jcs.01114. Epub 2004 May 11.
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