Zhao W, Shen H, Yuan F, Li G, Sun Y, Shi Z, Zhang Y, Wang Z
Beijing Neurosurgical Institute, Capital Medical University, Beijing.
Eur J Histochem. 2009 Mar 31;53(1):e7. doi: 10.4081/ejh.2009.e7.
The anterior pituitary gland undergoes tumourigenic changes in response to oestrogen treatment in several breeds of rats. We administered diethylstilbestrol (DES) to female Wistar rats and assessed whether the expression of vascular endothelial growth factor (VEGF) and aquaporin-1 (AQP-1) was altered at different time points following DES administration. magnetic resonance imaging (MRI) scans showed that the mass index corresponding to the mid-sagittal area of DES-treated pituitary was significantly higher than the vehicle-controlled pituitary (<0.01) at three specific time points, accompanied by a significant reduction in body weight. Haematoxylin and eosin (HE) staining and immunohistochemical analysis demonstrated that during early stages of induction, DES increased cell proliferation and sprouting of endothelial cells, and VEGF expression transitioned from a vessel-surrounding pattern to a diffuse pattern. During later stages, angiogenesis was predominant, and VEGF expression decreased. In contrast to the early abundant expression of VEGF, endothelial expression of AQP-1 increased during later stages. Our data indicated a dynamic scenario of biological alterations in DES-treated pituitary tissue, in which VEGF and AQP-1 exert their functions at different stages of induction, and we provide novel insights into understanding oestrogen-related tumourigenesis in the anterior pituitary gland.
在几种品系的大鼠中，垂体前叶在雌激素处理后会发生致瘤性变化。我们给雌性Wistar大鼠施用己烯雌酚（DES），并评估在DES给药后的不同时间点，血管内皮生长因子（VEGF）和水通道蛋白-1（AQP-1）的表达是否发生改变。磁共振成像（MRI）扫描显示，在三个特定时间点，DES处理的垂体矢状面中部区域对应的肿块指数显著高于载体对照的垂体（<0.01），同时体重显著降低。苏木精和伊红（HE）染色及免疫组织化学分析表明，在诱导早期，DES增加了细胞增殖和内皮细胞的芽生，VEGF表达从血管周围模式转变为弥漫模式。在后期，血管生成占主导，VEGF表达下降。与早期VEGF的丰富表达相反，AQP-1的内皮表达在后期增加。我们的数据表明DES处理的垂体组织中存在生物变化的动态情况，其中VEGF和AQP-1在诱导的不同阶段发挥作用，并且我们为理解垂体前叶中雌激素相关的肿瘤发生提供了新的见解。
