Herbst Florian-Alexander, Jehmlich Nico, von Bergen Martin, Schmidt Frank
Department of Chemistry and Bioscience, Aalborg University, Aalborg East, Denmark.
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany.
Methods Mol Biol. 2018;1841:163-174. doi: 10.1007/978-1-4939-8695-8_12.
Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis.
通过氨基酸的同位素标记对微生物进行定量蛋白质组分析仍然是一项挑战，因为只有具有营养缺陷型特征的微生物才能以定量正确的方式将氨基酸嵌入其生物质中。在此，我们描述了一种针对广泛生物体中含硫氨基酸半胱氨酸和甲硫氨酸的同位素标记技术（用于定量的氨基酸硫稳定同位素标记，SULAQ）。这种代谢标记方法适用于基于凝胶和非凝胶的蛋白质分析。
