B 细胞淋巴瘤-2 样蛋白-12 与变应性慢性鼻-鼻窦炎中的辅助性 T 细胞 2 型炎症相关。

B cell lymphoma-2-like protein-12 association with T-helper 2 inflammation in chronic rhinosinusitis with allergy.

机构信息

Department of Otolaryngology, Head & Neck Surgery, the Second Hospital, Shanxi Medical University, Taiyuan, China.

Ear-Nose-Throat (ENT) Institute and Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.

出版信息

Int Forum Allergy Rhinol. 2018 Nov;8(11):1300-1307. doi: 10.1002/alr.22223. Epub 2018 Oct 3.

DOI:10.1002/alr.22223

PMID:30281927

Abstract

BACKGROUND

T-helper 2 (Th2) polarization plays a critical role in the pathogenesis of chronic rhinosinusitis (CRS) with accompanying nasal allergy. Recent studies indicate that B cell lymphoma-2-like protein-12 (Bcl2L12) is associated with immune dysregulation. The purpose of this study was to elucidate the role of Bcl2L12 in the pathogenesis of Th2 polarization of CRS patients.

METHODS

CRS patients with nasal allergy (CRSa) and without nasal allergy (CRSna) were recruited into this study. CD4 T cells were isolated from the blood samples of human subjects. A variety of immunologic molecular strategies were used to assess Th2 polarization and Bcl2L12 expression.

RESULTS

Twenty CRSa patients, 20 CRSna patients, and 20 healthy subjects were recruited into this study. High levels of immunoglobulin E (IgE), interleukin 4 (IL-4), IL-5, IL-13, and Bcl2L12 were detected in nasal extracts of CRSa patients, but not in CRSna patients. The levels of Bcl2L12 were positively correlated with Th2 cytokines. CD4 T cells from CRSa patients were prone to differentiate into Th2 cells, in which Bcl2L12 was required.

CONCLUSION

Bcl2L12 is positively correlated with Th2 cytokine levels in the nasal mucosa of CRSa patients. Bcl2L12 contributes to the Th2 polarization, which may be a novel therapeutic target in the treatment of CRSa.

摘要

背景

辅助性 T 细胞 2（Th2）极化在伴有鼻过敏的慢性鼻-鼻窦炎（CRS）发病机制中起着关键作用。最近的研究表明，B 细胞淋巴瘤-2 样蛋白 12（Bcl2L12）与免疫失调有关。本研究旨在阐明 Bcl2L12 在 CRS 患者 Th2 极化发病机制中的作用。

方法

本研究纳入了伴有鼻过敏（CRSa）和不伴有鼻过敏（CRSna）的 CRS 患者。从人血样中分离 CD4 T 细胞。采用多种免疫分子策略评估 Th2 极化和 Bcl2L12 表达。

结果

本研究纳入了 20 例 CRSa 患者、20 例 CRSna 患者和 20 例健康对照者。CRSa 患者鼻分泌物中检测到高水平的免疫球蛋白 E（IgE）、白细胞介素 4（IL-4）、IL-5、IL-13 和 Bcl2L12，但在 CRSna 患者中未检测到。Bcl2L12 水平与 Th2 细胞因子呈正相关。CRSa 患者的 CD4 T 细胞易于分化为 Th2 细胞，其中需要 Bcl2L12。