大鼠载脂蛋白B mRNA的克隆与调控
Cloning and regulation of rat apolipoprotein B mRNA.
作者信息
Matsumoto A, Aburatani H, Shibasaki Y, Kodama T, Takaku F, Itakura H
出版信息
Biochem Biophys Res Commun. 1987 Jan 15;142(1):92-9. doi: 10.1016/0006-291x(87)90455-4.
Recombinant cDNA clones that code for apolipoprotein B(apoB) were isolated from a rat liver cDNA library, using synthetic oligonucleotide probe derived from the sequence of human apoB cDNA. The nucleotide and deduced amino acid sequences of the rat apoB clone pRB5, 1.2 kb in length, showed 83% and 84% homology to those of human apoB. Northern blot analysis revealed that rat apoB cDNA probe cross-reacts with human and rabbit apoB mRNA sequences and the size of those mRNAs, approximately 15 kb long, were not discernibly different. In addition, apoB mRNA was abundant only in the liver and intestine. Finally, cholesterol feeding to rats for six weeks resulted in a several-fold increase in the level of apoB mRNA in the liver.
利用源自人载脂蛋白B（apoB）cDNA序列的合成寡核苷酸探针，从大鼠肝脏cDNA文库中分离出编码载脂蛋白B的重组cDNA克隆。长度为1.2 kb的大鼠apoB克隆pRB5的核苷酸序列和推导的氨基酸序列与人类apoB的核苷酸序列和氨基酸序列分别具有83%和84%的同源性。Northern印迹分析表明，大鼠apoB cDNA探针与人及兔的apoB mRNA序列发生交叉反应，且这些mRNA的大小约为15 kb，无明显差异。此外，apoB mRNA仅在肝脏和肠道中大量存在。最后，给大鼠喂食胆固醇六周导致肝脏中apoB mRNA水平增加了几倍。
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