抗冻蛋白的应用：来自日本鱼类的优质产品的实际应用。

Applications of Antifreeze Proteins: Practical Use of the Quality Products from Japanese Fishes.

机构信息

Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan.

出版信息

Adv Exp Med Biol. 2018;1081:321-337. doi: 10.1007/978-981-13-1244-1_17.

DOI:10.1007/978-981-13-1244-1_17

PMID:30288717

Abstract

Numerous embryonic ice crystals are generated in water at the moment of freezing. These crystals grow and merge together to form an ice block that can be generally observed. Antifreeze protein (AFP) is capable of binding to the embryonic ice crystals, inhibiting such an ice block formation. Fish-derived AFP additionally binds to membrane lipid bilayers to prolong the lifetime of cells. These unique abilities of AFP have been studied extensively for the development of advanced techniques, such as ice recrystallization inhibitors, freeze-tolerant gels, cell preservation fluids, and high-porosity ceramics, for which mass-preparation method of the quality product of AFP utilizing fish muscle homogenates made a significant contribution. In this chapter, we present both fundamental and advanced information of fish AFPs that have been especially discovered from mid-latitude sea area, which will provide a hint to develop more advanced techniques applicable in both medical and industrial fields.

摘要

大量的冰晶胚胎在水结冰的瞬间产生。这些晶体生长并融合在一起，形成一个通常可以观察到的冰块。抗冻蛋白（AFP）能够与冰晶胚胎结合，抑制这种冰块的形成。鱼类来源的 AFP 还可以与膜脂双层结合，延长细胞的寿命。这些 AFP 的独特能力已经被广泛研究，以开发先进的技术，如冰晶再结晶抑制剂、耐冻凝胶、细胞保存液和高孔隙率陶瓷，为此，利用鱼类肌肉匀浆大规模制备 AFP 质量产品的方法做出了重大贡献。在本章中，我们介绍了从中纬度海域特别发现的鱼类 AFP 的基础和先进信息，这将为开发更先进的技术提供线索，这些技术在医学和工业领域都有应用。