Kim Yangseon, Kang In Jeong, Shin Dong Bum, Roh Jae Hwan, Heu Sunggi, Shim Hyeong Kwon
Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon, Korea.
Mycobiology. 2018 Aug 13;46(3):283-286. doi: 10.1080/12298093.2018.1496637. eCollection 2018.
causes the devastating plant disease head blight and produces mycotoxins on small cultivated grains. To investigate the timeframe of infection during rice cultivation, a spore suspension of was applied to the rice cultivars Dongjin 1 and Nampyeongbyeo before and after the heading stage. The disease incidence rate was the highest (50%) directly after heading, when the greatest number of flowers were present, while only 10% of the rice infected 30 days after heading showed symptoms. To understand the mechanism of infection, an strain expressing green fluorescent protein (GFP) was inoculated, and the resulting infections were visually examined. Spores were found in all areas between the glume and inner seed, with the largest amount of GFP detected in the aleurone layer. When the inner part of the rice seed was infected, the pathogen was mainly observed in the embryo. These results suggest that migrates from the anthers to the ovaries and into the seeds during the flowering stage of rice. This study will contribute to uncovering the infection process of this pathogen in rice.
引发毁灭性的植物病害小麦赤霉病，并在小粒栽培谷物上产生霉菌毒素。为了研究水稻种植过程中的感染时间框架，在抽穗期前后，将 的孢子悬浮液施用于水稻品种东津1号和南坪白米。发病率在抽穗后直接达到最高（50%），此时花朵数量最多，而抽穗30天后感染的水稻只有10%出现症状。为了解感染机制，接种了一株表达绿色荧光蛋白（GFP）的 菌株，并对由此产生的感染进行了肉眼检查。在颖片和内种子之间的所有区域都发现了孢子，在糊粉层中检测到的绿色荧光蛋白量最大。当水稻种子内部被感染时，病原体主要在胚中观察到。这些结果表明， 在水稻开花期从花药迁移到子房并进入种子。本研究将有助于揭示这种病原体在水稻中的感染过程。
