German Institute of Food Technologies (DIL e.V.), Quakenbrueck, Germany.
Leibniz Universitaet Hannover, Institute of Food Chemistry, Callinstr. 5, Hannover, Germany.
J Sci Food Agric. 2019 Mar 30;99(5):2175-2185. doi: 10.1002/jsfa.9410. Epub 2018 Nov 27.
High moisture extrusion (HME) of lupin protein concentrate and isolate (50:50) mixture was performed by varying the extrusion parameters, such as barrel temperature (138-180 °C), water feed (40-68%) and screw speed (400-1800 rpm). The effect of extrusion parameters on extruder responses [die pressure, product temperature, torque and specific mechanical energy (SME)] and product properties [colour, cutting force, cooking yield, microstructure and in vitro protein digestibility (IVPD)] was evaluated.
The multiple regression analysis of the results revealed that the water feed had a significant negative linear effect on the extruder responses considered, as well as on colour difference and cutting force of extrudates. Screw speed had a positive linear effect on product temperature, SME and cooking yield. Barrel temperature affected extruder responses and product properties to a lesser extent. Scanning electron microscopy showed that denser microstructure and higher number of fibre layers were created by increasing temperature and screw speed along with decreasing water feed. The results of IVPD of selected extrudates showed that the increase in barrel temperature decreased the IVPD, whereas the increase in water feed resulted in higher IVPD. The screw speed had no significant effect on IVPD.
The study demonstrates that the use of lupin protein is feasible to produce meat analogues with HME which could enhance the possibilities to meet the growing protein demands for human consumption. © 2018 Society of Chemical Industry.
通过改变挤压参数(如机筒温度(138-180°C)、水进料(40-68%)和螺杆速度(400-1800rpm))对羽扇豆蛋白浓缩物和分离物(50:50)混合物进行高水分挤压(HME)。评估了挤压参数对挤压机响应[模具压力、产品温度、扭矩和比机械能(SME)]和产品性能[颜色、切割力、蒸煮产率、微观结构和体外蛋白质消化率(IVPD)]的影响。
结果的多元回归分析表明,水进料对所考虑的挤压机响应以及色差和挤出物的切割力具有显著的负线性影响。螺杆速度对产品温度、SME 和蒸煮产率有正线性影响。机筒温度对挤压机响应和产品性能的影响较小。扫描电子显微镜显示,通过增加温度和螺杆速度并降低水进料量,形成了更致密的微观结构和更多的纤维层。对选定的挤出物进行 IVPD 的结果表明,机筒温度的升高降低了 IVPD,而水进料的增加导致 IVPD 更高。螺杆速度对 IVPD 没有显著影响。
该研究表明,使用羽扇豆蛋白生产 HME 肉类似物是可行的,这可以提高满足人类消费不断增长的蛋白质需求的可能性。©2018 化学工业协会。