庆大霉素对大鼠肾皮质的生化效应。I. 对照组织的分析性亚分级分离

Biochemical effects of gentamicin on rat kidney cortex. I. Analytical subfractionation of control tissue.

作者信息

Nässberger L, Bergstrand A, DePierre J W

出版信息

Exp Mol Pathol. 1987 Apr;46(2):217-29. doi: 10.1016/0014-4800(87)90067-0.

Abstract

As a first step in studies of the molecular mechanism(s) underlying gentamicin toxicity, rat kidney cortex has been subfractionated using differential centrifugation. An analytical, rather than preparative approach was used. DNA was used as a marker for the nuclei, cytochrome oxidase for mitochondria, acid phosphatase for lysosomes, catalase for peroxisomes, NADPH-cytochrome c reductase for the endoplasmic reticulum, p-nitrophenyl-alpha-mannosidase (at pH 5.5) for the Golgi apparatus, AMPase for the plasma membrane in general, and alkaline phosphatase for the brush border, and lactate dehydrogenase for the cytosol. In addition, electron microscopy was performed on the subfractions obtained. The distributions of subcellular markers obtained here for the rat kidney cortex closely resemble the corresponding distributions reported for rat liver. This procedure can now be used to look for biochemical and/or toxic changes which might be reflected in an altered distribution pattern for marker enzymes.

摘要

作为研究庆大霉素毒性潜在分子机制的第一步，已使用差速离心法对大鼠肾皮质进行了亚分级分离。采用的是分析方法而非制备方法。用DNA作为细胞核的标志物，细胞色素氧化酶作为线粒体的标志物，酸性磷酸酶作为溶酶体的标志物，过氧化氢酶作为过氧化物酶体的标志物，NADPH - 细胞色素c还原酶作为内质网的标志物，对硝基苯基 - α - 甘露糖苷酶（在pH 5.5时）作为高尔基体的标志物，AMP酶作为一般质膜的标志物，碱性磷酸酶作为刷状缘的标志物，乳酸脱氢酶作为胞质溶胶的标志物。此外，对获得的亚分级分离物进行了电子显微镜检查。此处获得的大鼠肾皮质亚细胞标志物的分布与报道的大鼠肝脏的相应分布非常相似。该程序现在可用于寻找可能反映在标志物酶分布模式改变中的生化和/或毒性变化。