桑黄花叶病毒核心颗粒形成于富含病毒磷蛋白的环状核结构上。

Sonchus yellow net virus core particles form on ring-like nuclear structure enriched in viral phosphoprotein.

机构信息

Molecular and Cellular Imaging Center, Department of Plant Pathology, The Ohio State University, Wooster, OH, 44691, USA.

USDA-ARS Plant Pathology, 1680 Madison Ave, Wooster, OH, 44691, USA.

出版信息

Virus Res. 2018 Oct 15;258:64-67. doi: 10.1016/j.virusres.2018.10.005. Epub 2018 Oct 9.

DOI:10.1016/j.virusres.2018.10.005

PMID:30308212

Abstract

The phosphoprotein (P) of the nucleorhabdovirus sonchus yellow net virus has been shown to accumulate in ring-shaped structures in virus-infected nuclei. Further examination by live-cell imaging, in combination with structural examination by transmission electron microscopy and immunolocalization demonstrated that P-rings do not form in association with nucleoli. Furthermore, viral cores were shown to condense on the nucleoplasm-contacting surface of the rings. The data presented here offer evidence for the site of nucleocapsid assembly in SYNV-infected nuclei.

摘要

核质弹状病毒（sonchus yellow net virus）的磷蛋白（P）已被证明在病毒感染的细胞核中积累成环形结构。通过活细胞成像进一步检查，结合透射电子显微镜结构检查和免疫定位表明，P 环不是与核仁相关形成的。此外，还显示病毒核心在环的核质接触面处浓缩。这里提供的资料为 SYNV 感染核中核衣壳组装的部位提供了证据。