两种富含脯氨酸唾液蛋白家族（aPRP 和 bPRP）混合物对与葡萄酒黄烷醇相互作用的协同效应。
Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols.
机构信息
Grupo de Investigación en Polifenoles (GIP), Facultad de Farmacia, University of Salamanca, Salamanca, Spain.
Grupo de Investigación en Polifenoles (GIP), Facultad de Farmacia, University of Salamanca, Salamanca, Spain.
出版信息
Food Chem. 2019 Jan 30;272:210-215. doi: 10.1016/j.foodchem.2018.08.024. Epub 2018 Aug 8.
In this study, we have evaluated by HPLC-DAD, DLS and MALDI-TOF a synergic effect of the coexistence of two salivary-PRP fractions (basic-PRPs and acidic PRPs) on the interaction with flavanols. Results obtained showed noticeable enhancement of the interaction between (epi)catechin and PRPs when both types of proteins are blended. Up to 30 soluble aggregates have been tentatively identified with molecular weight from 4680 to 35,851. (epi)Catechins seem to bind preferentially bPRPs than aPRPs, although the medium size aggregates flavanol-bPRPs formed could favour the interaction with aPRPs giving rise to soluble mixed aggregates.
在这项研究中，我们通过 HPLC-DAD、DLS 和 MALDI-TOF 评估了两种唾液 PRP 级分（碱性 PRP 和酸性 PRP）共存对与黄烷醇相互作用的协同效应。结果表明，当两种蛋白质混合时，（表）儿茶素与 PRP 之间的相互作用明显增强。已经初步鉴定出多达 30 种可溶性聚集体，分子量从 4680 到 35851 不等。（表）儿茶素似乎优先与 bPRP 结合，而不是与 aPRP 结合，尽管形成的中等大小黄烷醇-bPRP 聚集体可能有利于与 aPRP 相互作用，从而产生可溶性混合聚集体。
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