免疫反应蛋白的抗原决定簇：烯醇化酶、肌苷 5'-单磷酸脱氢酶和分子伴侣 GroEL。
Epitopes of Immunoreactive Proteins of : Enolase, Inosine 5'-Monophosphate Dehydrogenase and Molecular Chaperone GroEL.
机构信息
Department of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland.
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
出版信息
Front Cell Infect Microbiol. 2018 Oct 2;8:349. doi: 10.3389/fcimb.2018.00349. eCollection 2018.
Three (group B streptococci, GBS) immunoreactive proteins: enolase (47.4 kDa), inosine 5'-monophosphate dehydrogenase (IMPDH) (53 kDa) and molecular chaperone GroEL (57 kDa) were subjected to investigation. Enolase protein was described in our previous paper, whereas IMPDH and GroEL were presented for the first time. The aim of our paper was to provide mapping of specific epitopes, highly reactive with umbilical cord blood serum. Bioinformatic analyses allowed to select 32 most likely epitopes for enolase, 36 peptides for IMPDH and 41 immunoreactive peptides for molecular chaperone GroEL, which were synthesized by PEPSCAN. Ten peptides: two in enolase, one in IMPDH and seven in molecular chaperone GroEL have been identified as potentially highly selective epitopes that can be used as markers in rapid immunological diagnostic tests or constitute a component of an innovative vaccine against GBS infections.
三种（B 群链球菌，GBS）免疫反应性蛋白：烯醇化酶（47.4 kDa）、肌苷 5'-单磷酸脱氢酶（IMPDH）（53 kDa）和分子伴侣 GroEL（57 kDa）进行了研究。烯醇化酶蛋白在我们之前的论文中已有描述，而 IMPDH 和 GroEL 则是首次提出。我们论文的目的是提供与脐血血清高度反应的特异性表位作图。生物信息学分析允许选择 32 个最可能的烯醇化酶表位、36 个 IMPDH 肽和 41 个分子伴侣 GroEL 的免疫反应性肽，这些肽由 PEPSCAN 合成。已鉴定出 10 种肽：烯醇化酶中的 2 种、IMPDH 中的 1 种和分子伴侣 GroEL 中的 7 种，它们可能是高度选择性的表位，可以用作快速免疫诊断试验的标志物，或者构成针对 GBS 感染的创新疫苗的组成部分。
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