纯度高且功能强大的大鼠骨髓源性肥大细胞的短期体外培养
Short-term in vitro culture of purity and highly functional rat bone marrow-derived mast cells.
作者信息
Yu Tianyu, Liu Bin, He Zhigang, Yang Muqing, Song Jian, Ma Cheng, Ma Sunqiang, Feng Junlan, Wang Xiaodong, Li Jiyu
机构信息
Department of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, 81 MeShan Road, Hefei, 230032, Anhui, People's Republic of China.
Department of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, People's Republic of China.
出版信息
In Vitro Cell Dev Biol Anim. 2018 Dec;54(10):705-714. doi: 10.1007/s11626-018-0301-3. Epub 2018 Oct 19.
Mast cells (MCs) are responsible for the innate immune response. Rat MCs are more suitable than mouse MCs as models of specific parasite infection processes and ovalbumin-induced asthma. Rat peritoneum-derived MCs and RBL-2H3 cells (an MC cell line) are widely used in disease studies. However, the application of rat bone marrow-derived MCs (BMMCs) are poorly documented in terms of the methodology of rat BMMC isolation. Here, we describe a relatively rapid, efficient, and simple method for the cultivation of rat BMMCs. As compared to previous protocols, rat BMMCs produced with the proposed protocol exhibited advantages in differentiation, proliferation, lifespan, and functionality, which should prove useful for studies of mucosal MC diseases in specific rat models.
肥大细胞（MCs）负责先天免疫反应。作为特定寄生虫感染过程和卵清蛋白诱导哮喘的模型，大鼠MCs比小鼠MCs更合适。大鼠腹膜来源的MCs和RBL-2H3细胞（一种MC细胞系）广泛用于疾病研究。然而，关于大鼠骨髓来源的MCs（BMMCs）的分离方法，其应用的文献报道较少。在此，我们描述了一种相对快速、高效且简单的大鼠BMMCs培养方法。与先前的方案相比，用所提出的方案产生的大鼠BMMCs在分化、增殖、寿命和功能方面表现出优势，这对于特定大鼠模型中黏膜MC疾病的研究应是有用的。
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