Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Cell Sheet Tissue Engineering Center and Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Lake City, UT, USA.
Adv Exp Med Biol. 2018;1078:371-393. doi: 10.1007/978-981-13-0950-2_19.
This chapter describes the concept of "cell sheet engineering" for the creation of transplantable cellular tissues and organs. In contrast to scaffold-based tissue engineering, cell sheet engineering facilitates the reconstruction of scaffold-free, cell-dense tissues. Cell sheets were harvested by changing the temperature of thermoresponsive cell culture surfaces modified with poly(N-isopropylacrylamide) (PIPAAm) with a thickness on the nanometer scale. The transplantation of 2D cell sheet tissues has been used in clinical settings. Although 3D tissues were formed simply by layering 2D cell sheets, issues related to vascularization within 3D tissues and the large-scale production of cells must be addressed to create thick and large 3D tissues and organs.
本章介绍了用于创建可移植细胞组织和器官的“细胞片工程”概念。与基于支架的组织工程相比，细胞片工程有助于构建无支架、细胞密集的组织。通过改变纳米级厚度的聚（N-异丙基丙烯酰胺）（PIPAAm）修饰的温敏细胞培养表面的温度来收获细胞片。二维细胞片组织的移植已在临床环境中使用。尽管通过层叠二维细胞片可以简单地形成三维组织，但必须解决与三维组织内血管生成和大量细胞生产相关的问题，以创建厚大和大体积的三维组织和器官。
