Macchi B, Popovic M, Allavena P, Rossi P, Gallo R C, Bonmassar E
Int J Tissue React. 1987;9(3):195-8.
Infection with human T-cell leukaemia/lymphoma (HTLV-I) preferentially affects T cells of the OKT-4 phenotype. The aim of the present study was to determine whether distinct T-cell subsets exhibit differences in susceptibility to virus infection. T cells from peripheral blood were separated according to cell densities by 7-step Percoll gradients. Separated T-cell subpopulations were infected with HTLV-I, using cocultivation with irradiated virus producer MT-2 cell line. Percentages of HTLV-I-infected cells and their phenotypes were assayed by immunofluorescence assay (IFA), using highly specific mouse monoclonal antibody directed against HTLV-I P-19 core protein, and other surface markers. The results showed that different T-cell subpopulations were susceptible to HTLV-I infection with the exception of large granular lymphocytes (LGL) which exhibit high cell-mediated natural cytotoxicity (CMNC).
人类T细胞白血病/淋巴瘤病毒1型（HTLV-I）感染优先影响OKT-4表型的T细胞。本研究的目的是确定不同的T细胞亚群在病毒感染易感性方面是否存在差异。通过7步Percoll梯度根据细胞密度分离外周血中的T细胞。使用与经辐照的病毒产生细胞系MT-2共培养的方法，将分离出的T细胞亚群用HTLV-I感染。采用针对HTLV-I P-19核心蛋白和其他表面标志物的高度特异性小鼠单克隆抗体，通过免疫荧光测定法（IFA）检测HTLV-I感染细胞的百分比及其表型。结果表明，除了表现出高细胞介导的自然细胞毒性（CMNC）的大颗粒淋巴细胞（LGL）外，不同的T细胞亚群对HTLV-I感染均易感。
