Kim C H, Balny C, King T E
J Biol Chem. 1987 Jun 15;262(17):8103-8.
A role of the hinge protein is studied in the electron transfer reaction between cytochromes c1 and c, using highly purified "one-band" cytochrome c1 and "two-band" cytochrome c1. The results show that the hinge protein (Hp), which is essential for a stable ionic strength-sensitive c1-Hp-c complex, seems to play a certain role in electron transfer between cytochromes c1 and c; Keq for electron transfer reaction between cytochromes c1 and c in the presence of the hinge protein is found to be about 40% higher than that in the absence of the hinge protein at low ionic strength, but no difference exists at high ionic strength. We propose a hypothesis that the hinge protein may function as regulator for the electron transfer reaction between cytochromes c1 and c, and this may be at least one of the roles of the hinge protein in mitochondria.
利用高度纯化的“单带”细胞色素c1和“双带”细胞色素c1，研究了铰链蛋白在细胞色素c1和c之间的电子转移反应中的作用。结果表明，对于稳定的离子强度敏感的c1-Hp-c复合物至关重要的铰链蛋白（Hp）似乎在细胞色素c1和c之间的电子转移中起一定作用；发现在低离子强度下，存在铰链蛋白时细胞色素c1和c之间电子转移反应的平衡常数（Keq）比不存在铰链蛋白时高约40%，但在高离子强度下没有差异。我们提出一个假设，即铰链蛋白可能作为细胞色素c1和c之间电子转移反应的调节剂发挥作用，这可能是铰链蛋白在线粒体中的至少一个作用。
