Prova Ananya, Akanda Abdul Mannan, Islam Shaikhul, Hossain Md Motaher
Department of Plant Pathology, EXIM Bank Agricultural University, Chapainawabganj-6300, Bangladesh.
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh.
Plant Pathol J. 2018 Oct;34(5):367-380. doi: 10.5423/PPJ.OA.02.2018.0028. Epub 2018 Oct 1.
Stems and pods of hyacinth bean cultivated in a farmer's field in Gazipur District, Bangladesh, were found rotted in nearly 5% hyacinth bean plants. A fungus having fluffy mycelium and large sclerotia was isolated from affected tissues. Combined results of morphological, molecular and pathological analyses identified the fungus as (Lib) de Bary. Inoculating the fungus on healthy hyacinth bean plants and pods reproduced the symptoms previously observed in the field. The three isolates obtained from naturally infected plants were cross inoculated in hyacinth bean, okra and African-American marigold and they were pathogenic to these hosts. The optimum temperature and pH for its growth were 20°C and pH 5.0, respectively. Sclerotial development was favored at pH 5.0. Sucrose and mannitol were the best carbon sources to support hyphal growth, while glucose was the most favourable for sclerotial development. The hyacinth bean genotypes, HB-82 (Rupban Sheem) and HB-102 were found highly resistant, while HB-94 (Ashina) was moderate resistant to the fungus. Finally, was sensitive to Bavistin, Dithane M-45 and Rovral fungicides and Ca in the form of CaCl. This observation could possibly aid in eliminating field loss in hyacinth bean caused by an emerging pathogenic fungus .
在孟加拉国加济布尔区一位农民田地里种植的扁豆茎和豆荚，在近5%的扁豆植株中发现腐烂。从受影响的组织中分离出一种具有绒毛状菌丝体和大菌核的真菌。形态学、分子学和病理学分析的综合结果确定该真菌为德巴利腐霉（Lib）。将该真菌接种到健康的扁豆植株和豆荚上，再现了之前在田间观察到的症状。从自然感染植株中获得的三个分离株在扁豆、秋葵和非洲万寿菊上进行交叉接种，它们对这些寄主具有致病性。其生长的最适温度和pH分别为20°C和pH 5.0。在pH 5.0时有利于菌核发育。蔗糖和甘露醇是支持菌丝生长的最佳碳源，而葡萄糖对菌核发育最有利。发现扁豆基因型HB - 82（鲁班希姆）和HB - 102对该真菌高度抗性，而HB - 94（阿希纳）中度抗性。最后，德巴利腐霉对多菌灵、代森锰锌和农利灵杀菌剂以及氯化钙形式的钙敏感。这一观察结果可能有助于消除由一种新出现的致病真菌德巴利腐霉造成的扁豆田间损失。
