Key Lab of Agriculture Animal Genetics, Breeding, & Reproduction of Ministry of Education, College of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Guangzhou Key Laboratory of Insect Development Regulation & Application Research, Institute of Insect Science & Technology & School of Life Sciences, South China Normal University, Guangzhou, 510631, PR China.
Epigenomics. 2018 Dec;10(12):1603-1618. doi: 10.2217/epi-2017-0117. Epub 2018 Oct 29.
We aimed to identify previously unreported long intergenic noncoding RNAs (lincRNAs) in the porcine liver, an important metabolic tissue, and further illustrate the epigenomic landscapes and the evolution of lincRNAs.
MATERIALS & METHODS: We used porcine omics data and comprehensively analyzed and identified lincRNAs and their methylation, expression and evolutionary patterns during pig domestication.
LincRNAs exhibit highly methylated promoter and downstream regions, as well as lower expression levels and higher tissue specificity than protein-coding genes. We identified a batch of lincRNAs with selection signals that are associated with pig domestication, which are more highly expressed in the liver than in other tissues (19:10/8/6/3/2/1/1). Interestingly, the lincRNA linc-sscg1779 and its target gene C6, which is crucial in liver metabolism, are differentially expressed during pig domestication.
Although they may originate from noisy transcripts, lincRNAs may be subjected to artificial selection. This phenomenon implies the functional importance of lincRNAs in pig domestication.
我们旨在鉴定猪肝脏这一重要代谢组织中以前未报道的长链非编码 RNA（lncRNA），并进一步阐述 lncRNA 的表观基因组景观和进化。
我们使用猪组学数据，全面分析和鉴定了 lncRNA 及其在猪驯化过程中的甲基化、表达和进化模式。
lncRNA 表现出高度甲基化的启动子和下游区域，以及比蛋白质编码基因更低的表达水平和更高的组织特异性。我们鉴定了一批与猪驯化相关的具有选择信号的 lincRNA，它们在肝脏中的表达水平高于其他组织（19:10/8/6/3/2/1/1）。有趣的是，lncRNA linc-sscg1779 及其在肝脏代谢中至关重要的靶基因 C6 在猪驯化过程中呈现差异表达。
尽管它们可能起源于嘈杂的转录本，但 lncRNA 可能受到人工选择。这种现象表明 lncRNA 在猪驯化过程中的功能重要性。
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