小型猪循环死亡后捐赠（DCD）供肝模型的综合评估
Comprehensive Evaluation of a Donated After Circulatory Death (DCD) Donor Liver Model in Minipigs.
作者信息
Wang Guang, Cheng Ying, Liu Yongfeng
机构信息
Department of Hepatobiliary Surgery and Organ Transplantation, The First Hospital of China Medical University, Shenyang, Liaoning, China (mainland).
出版信息
Ann Transplant. 2018 Oct 30;23:758-766. doi: 10.12659/AOT.911072.
BACKGROUND Graft livers from donors after death from circulatory disease (DCD) often suffer injury from severe ischemia or hypoxia before resection. Thus, earlier evaluation of these livers is critical for the survival of recipients. MATERIAL AND METHODS In our study, 18 minipigs, as DCD donor liver models, were evenly divided into a warm ischemia time (WIT) group and a hypoxia plus ischemia group. Another 18 minipigs served as recipients and were implanted with the donor livers of the DCD models. miR-122 levels and hepatic function were examined before and after liver transplantation. RESULTS Results indicated that increased miR-122 levels appeared in the early stages of ischemia and hypoxia. Increases in ALT and GGT levels occurred earlier than changes in TBil. CONCLUSIONS The expression levels of miR-122 in donor liver might play a role in the evaluation of organ injury. Changes in donor liver functions were more sensitive to ischemia than hypoxia in this established porcine DCD model.
背景 来自循环系统疾病死亡供体（DCD）的移植肝脏在切除前常遭受严重缺血或缺氧损伤。因此，对这些肝脏进行早期评估对受体的存活至关重要。材料与方法 在我们的研究中，18只小型猪作为DCD供肝模型，被均匀分为热缺血时间（WIT）组和缺氧加缺血组。另外18只小型猪作为受体，植入DCD模型的供肝。在肝移植前后检测miR-122水平和肝功能。结果 结果表明，在缺血和缺氧早期miR-122水平升高。ALT和GGT水平的升高比TBil的变化出现得更早。结论 供肝中miR-122的表达水平可能在器官损伤评估中起作用。在这个建立的猪DCD模型中，供肝功能的变化对缺血比缺氧更敏感。
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