通过 BACCS 对细胞内钙的光遗传学操作促进 MC3T3-E1 细胞的分化。
Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells.
机构信息
Department of Orthodontic Science, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan; Department of Cell Biology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan; The Center for Brain Integration Research (CBIR), Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
Department of Cell Biology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan; The Center for Brain Integration Research (CBIR), Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
出版信息
Biochem Biophys Res Commun. 2018 Nov 30;506(3):716-722. doi: 10.1016/j.bbrc.2018.10.107. Epub 2018 Oct 27.
Bone remodeling is maintained through the balance between bone formation by osteoblasts and bone resorption by osteoclasts. Previous studies suggested that intracellular Ca signaling plays an important role in the differentiation of osteoblasts; however, the molecular mechanism of Ca signaling in the differentiation of osteoblasts remains unclear. To elucidate the effect of Ca signaling in osteoblasts, we employed an optogenetic tool, blue light-activated Ca channel switch (BACCS). BACCS was used to spatiotemporally control intracellular Ca with blue light stimulation. MC3T3-E1 cells, which have been used as a model of differentiation from preosteoblast to osteoblast, were promoted to differentiate by BACCS expression and rhythmical blue light stimulation. The results indicated that intracellular Ca change from the outside of the cells can regulate signaling for differentiation of MC3T3-E1 cells. Our findings provide evidence that Ca could cause osteoblast differentiation.
骨重建通过成骨细胞形成骨和破骨细胞吸收骨之间的平衡来维持。先前的研究表明，细胞内 Ca 信号在成骨细胞的分化中起着重要作用；然而，成骨细胞分化中 Ca 信号的分子机制仍不清楚。为了阐明 Ca 信号在成骨细胞中的作用，我们使用了一种光遗传学工具，即蓝光激活 Ca 通道开关（BACCS）。BACCS 被用来通过蓝光刺激时空控制细胞内 Ca。MC3T3-E1 细胞已被用作从前成骨细胞分化为成骨细胞的模型，通过 BACCS 表达和节律性蓝光刺激促进其分化。结果表明，细胞外的 Ca 变化可以调节 MC3T3-E1 细胞分化的信号。我们的发现提供了证据表明 Ca 可以引起成骨细胞分化。
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