Wahl S M, McCartney-Francis N, Hunt D A, Smith P D, Wahl L M, Katona I M
J Immunol. 1987 Aug 15;139(4):1342-7.
Activation of human peripheral blood monocytes results in the expression of interleukin 2 (IL 2) receptors, which are absent on resting monocytes. In a population of purified monocytes, the appearance of IL 2 receptors occurs on the majority of cells in association with increased levels of HLA-DR. Lipopolysaccharide (LPS) induces maximum numbers of IL 2 receptors within 12 hr, whereas IFN-gamma requires 48 hr. We used cDNA encoding for the human IL 2 receptor to evaluate IL 2 receptor gene expression in resting and activated monocytes. Within 4 hr after LPS stimulation, IL 2 receptor mRNA species of 3500 and 1500 bases appear, reaching peak levels between 8 and 12 hr and declining thereafter. The LPS-activated monocyte IL 2 receptor protein is expressed on the cell surface within a few hours after the detection of IL 2 receptor mRNA. The addition of IL 2 to IL 2 receptor-positive monocytes augments their generation of reactive oxygen intermediates and their cytotoxic activity. Thus monocytes when activated undergo a series of morphologic, phenotypic, and functional changes, including the expression of IL 2 receptors, which may provide an important immunoregulatory pathway.
人外周血单核细胞的激活会导致白细胞介素2（IL-2）受体的表达，而静息单核细胞上不存在该受体。在纯化的单核细胞群体中，IL-2受体出现在大多数细胞上，同时伴有HLA-DR水平的升高。脂多糖（LPS）在12小时内诱导出最大数量的IL-2受体，而干扰素-γ则需要48小时。我们使用编码人IL-2受体的cDNA来评估静息和激活的单核细胞中IL-2受体基因的表达。在LPS刺激后4小时内，出现了3500和1500个碱基的IL-2受体mRNA种类，在8至12小时达到峰值水平，此后下降。LPS激活的单核细胞IL-2受体蛋白在检测到IL-2受体mRNA后的几小时内在细胞表面表达。将IL-2添加到IL-2受体阳性的单核细胞中会增强其活性氧中间体的生成及其细胞毒性活性。因此，单核细胞激活后会经历一系列形态、表型和功能变化，包括IL-2受体的表达，这可能提供一条重要的免疫调节途径。
