鞘氨醇单胞菌中N-酰基高丝氨酸内酯（AHL）降解活性及AHL内酯酶基因（qsdS）的分布与特性分析
Distribution and characterization of N-acylhomoserine lactone (AHL)-degrading activity and AHL lactonase gene (qsdS) in Sphingopyxis.
作者信息
Morohoshi Tomohiro, Kamimura Yaoki, Sato Niina, Iizumi Taro
机构信息
Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi 321-8585, Japan.
Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi 321-8585, Japan.
出版信息
J Biosci Bioeng. 2019 Apr;127(4):411-417. doi: 10.1016/j.jbiosc.2018.10.005. Epub 2018 Oct 31.
N-Acylhomoserine lactone (AHL)-degrading enzyme is identified from the various environments and applied for quorum-sensing inhibition. In this study, we isolated two AHL-degrading strains, Sphingopyxis sp. EG6 and FD7, from the industrial cooling water samples. When the eight Sphingopyxis type strains were checked for the AHL-degrading activity, two strains, Sphingopyxis alaskensis DSM 13593 and Sphingopyxis bauzanensis DSM 22271, showed high AHL-degrading activity. The complete genome sequences of EG6 and FD7 revealed the presence of gene homolog of qsdS, which encodes AHL-lactonase in Sphingomonas ursincola. The qsdS gene is seated between putative gene homologs involved in 3-isopropylmalate dehydratase large (leuC2) and small (leuD) subunits in the genome of EG6, FD7, DSM 13593, and DSM 22271, but completely disappeared between leuC2 and leuD in the genome sequences of Sphingopyxis type strains without AHL-degrading activity. Purified His-tagged QsdS showed high AHL-degrading activity and catalyzed AHL ring opening by hydrolyzing lactones. In addition, heterologous expression of qsdS in Pseudomonas aeruginosa resulted in reduction of biofilm formation. These results suggested that the AHL-degrading activity in Sphingopyxis is useful as an effective agent for biofilm inhibition.
N-酰基高丝氨酸内酯（AHL）降解酶是从各种环境中鉴定出来的，并应用于群体感应抑制。在本研究中，我们从工业冷却水样品中分离出两株AHL降解菌株，鞘氨醇单胞菌属（Sphingopyxis sp.）EG6和FD7。当检测八株鞘氨醇单胞菌属模式菌株的AHL降解活性时，两株菌株，阿拉斯加鞘氨醇单胞菌（Sphingopyxis alaskensis）DSM 13593和包赞鞘氨醇单胞菌（Sphingopyxis bauzanensis）DSM 22271，表现出高AHL降解活性。EG6和FD7的全基因组序列显示存在qsdS的基因同源物，该基因在栖 Ursincola鞘氨醇单胞菌中编码AHL内酯酶。qsdS基因位于EG6、FD7、DSM 13593和DSM 22271基因组中参与3-异丙基苹果酸脱水酶大亚基（leuC2）和小亚基（leuD）的假定基因同源物之间，但在没有AHL降解活性的鞘氨醇单胞菌属模式菌株的基因组序列中，该基因在leuC2和leuD之间完全消失。纯化的His标签QsdS显示出高AHL降解活性，并通过水解内酯催化AHL开环。此外，qsdS在铜绿假单胞菌中的异源表达导致生物膜形成减少。这些结果表明，鞘氨醇单胞菌属中的AHL降解活性可作为生物膜抑制的有效试剂。
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