通过选择性进化，一种用于区分 HSA 和 BSA 的荧光传感器。

A fluorescent sensor for discrimination of HSA from BSA through selectivity evolution.

机构信息

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.

出版信息

Anal Chim Acta. 2018 Dec 28;1043:123-131. doi: 10.1016/j.aca.2018.09.010. Epub 2018 Sep 8.

DOI:10.1016/j.aca.2018.09.010

PMID:30392660

Abstract

Pre-clinical diagnosis of many diseases required quantitative detection of Human Serum Albumin (HSA). Herein a high-selective HSA sensor RhHSA was picked through a two-round selectivity evolution from the typical "Effector-π-Trigger" style. RhHSA suggested advantages including high selective (∼6 fold for HSA:BSA = 1:10), sensitive (LOD ∼ 5 nM, over 700-fold enhancement), steady (over 24 h) and wide linear range (0-0.5 mg/mL, applicative for conventional HSA measurement). The detecting system was free from media polarity or viscosity. HSA destruction, site competition and molecular docking provided reliable evidence for the fact that RhHSA could be embedded into both ibuprofen and phenylbutazone sites of HSA. These hints also supported the discrimination of HSA from BSA. Stepwisely fluid replacement in living cells and measuring in urine system both inferred the potential of RhHSA in biological applications.

摘要

许多疾病的临床前诊断都需要定量检测人血清白蛋白（HSA）。在此，通过两轮选择性进化，从典型的“效应物-π-触发”风格中筛选出高选择性的 HSA 传感器 RhHSA。RhHSA 具有以下优点：高选择性（HSA：BSA=1:10 时约为 6 倍）、灵敏（LOD∼5 nM，增强超过 700 倍）、稳定（超过 24 h）和宽线性范围（0-0.5 mg/mL，适用于常规 HSA 测量）。检测系统不受介质极性或粘度的影响。HSA 破坏、位点竞争和分子对接为 RhHSA 可以嵌入 HSA 的布洛芬和苯丁唑酮位点这一事实提供了可靠的证据。这些线索也支持了 RhHSA 对 HSA 和 BSA 的区分。在活细胞中逐步替换流体并在尿液系统中进行测量，这都暗示了 RhHSA 在生物应用中的潜力。