Lerea K M, Glomset J A
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5620-4. doi: 10.1073/pnas.84.16.5620.
We studied the influence of prostaglandin E1 and theophylline on the ability of rabbit or human platelets to form NaDodSO4-resistant complexes between 125I-labeled thrombin and a platelet protein of approximately equal to 40 kDa. Control platelets formed two types of these complexes, one that sedimented with the platelets and one that was found in the suspension medium. There were 30-40 sedimentable complexes per platelet and about three times this number of soluble complexes. Pretreatment of rabbit or human platelets with prostaglandin E1 and theophylline decreased the formation of both types of complex by as much as 60-80%. The pretreatment was particularly effective when low doses of thrombin were used. When added to control platelets, such doses of thrombin caused the formation of sedimentable complexes and the concomitant formation of inositol trisphosphate. However, when added to platelets that had been pretreated with prostaglandin E1 and theophylline, low doses of thrombin had little or no effect on either reaction.
我们研究了前列腺素E1和茶碱对兔或人血小板在125I标记的凝血酶与一种分子量约为40 kDa的血小板蛋白之间形成耐十二烷基硫酸钠（NaDodSO4）复合物能力的影响。对照血小板形成了两种类型的这种复合物，一种与血小板一起沉淀，另一种存在于悬浮介质中。每个血小板有30 - 40个可沉淀复合物，可溶性复合物的数量约为其3倍。用前列腺素E1和茶碱对兔或人血小板进行预处理，可使两种类型复合物的形成减少多达60 - 80%。当使用低剂量凝血酶时，这种预处理尤为有效。当将此类剂量的凝血酶添加到对照血小板中时，会导致可沉淀复合物的形成以及伴随的肌醇三磷酸的形成。然而，当将低剂量凝血酶添加到已用前列腺素E1和茶碱预处理的血小板中时，对这两种反应几乎没有影响。
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