Central Laboratory, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong Province, People's Republic of China.
Central Laboratory, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong Province, People's Republic of China.
Fertil Steril. 2018 Nov;110(6):1058-1066. doi: 10.1016/j.fertnstert.2018.07.008.
To investigate the potential effects of TAT-PRDX2 protein supplementation to the cryopreservation medium on post-thaw sperm quality and function.
In vitro prospective study.
Medical university hospital.
PATIENT(S): Fifty normozoospermic, 50 asthenozoospermic, and 50 oligoasthenozoospermic men undergoing semen analysis for couple infertility.
INTERVENTION(S): Each semen sample was divided into three aliquots: fresh, cryopreserved control (without additive), and cryopreserved with TAT-PRDX2 protein.
MAIN OUTCOME MEASURE(S): Sperm motility, viability, mitochondrial potential, and DNA damage as well as reactive oxygen species (ROS) levels and lipid peroxidation were analyzed. Acrosome reaction and zona-free hamster oocyte penetration tests were performed to assess the fertilization ability of cryopreserved spermatozoa.
RESULT(S): In normozoospermic and asthenozoospermic groups, the addition of 150 μg/mL TAT-PRDX2 significantly reduced intracellular ROS and malondialdehyde levels and enhanced post-thaw sperm motility and viability when compared with the cryopreserved control of the respective groups but did not produce any significant protective effect in the oligoasthenozoospermic group. Mitochondrial potential was significantly increased, whereas DNA fragmentation was significantly decreased, after TAT-PRDX2 supplementation only in the asthenozoospermic group when compared with the cryopreserved control. Although the penetration rate and the penetration index were not markedly improved, TAT-PRDX2 supplementation obviously reduced spontaneous acrosome reaction and increased calcium ionophore-induced acrosome reaction in the normozoospermic and asthenozoospermic groups.
CONCLUSION(S): TAT-PRDX2 protein effectively exerted cryoprotective effects on spermatozoa by reducing intracellular ROS level and thereby improved post-thaw sperm quality and function, especially for asthenozoospermic samples. TAT-PRDX2 protein is a promising additive for developing a new and highly efficient semen cryoprotectant.
研究 TAT-PRDX2 蛋白添加到冷冻保存液中对解冻后精子质量和功能的潜在影响。
体外前瞻性研究。
医科大学医院。
50 名正常精子症、50 名弱精子症和 50 名少弱精子症患者，因夫妇不育进行精液分析。
每个精液样本分为三份：新鲜样本、无添加剂冷冻保存对照样本和添加 TAT-PRDX2 蛋白的冷冻保存样本。
分析精子活力、活力、线粒体潜能和 DNA 损伤以及活性氧（ROS）水平和脂质过氧化。进行顶体反应和去透明带仓鼠卵穿透试验，以评估冷冻保存精子的受精能力。
在正常精子症和弱精子症组中，与各自组的冷冻保存对照相比，添加 150μg/ml TAT-PRDX2 可显著降低细胞内 ROS 和丙二醛水平，并增强解冻后精子活力和活力，但在少弱精子症组中没有产生任何显著的保护作用。与冷冻保存对照相比，仅在弱精子症组中，TAT-PRDX2 补充后线粒体潜能显著增加，而 DNA 碎片化显著降低。虽然穿透率和穿透指数没有明显改善，但 TAT-PRDX2 补充明显减少了正常精子症和弱精子症组中的自发性顶体反应，并增加了钙离子载体诱导的顶体反应。
TAT-PRDX2 蛋白通过降低细胞内 ROS 水平有效发挥对精子的保护作用，从而改善解冻后精子质量和功能，尤其是对弱精子症样本。TAT-PRDX2 蛋白是开发新型高效精液冷冻保护剂的有前途的添加剂。
