Filatov I A, Kulish M A, Mironov A F, Nozhevnikova E V, Neĭman L A
Bioorg Khim. 1987 May;13(5):623-7.
Treatment of molecular crystals of the bovine cytochrome oxidase and the cytochrome oxidase-cytochrome c complex with thermally activated tritium leads to highly labelled cytochrome oxidase preparations. HPLC separation of its subunits and measurements of radioactivity of each polypeptide allow to determine the shielding of cytochrome oxidase surface sites by cytochrome c in the complex.
用热活化氚处理牛细胞色素氧化酶和细胞色素氧化酶 - 细胞色素c复合物的分子晶体，可得到高度标记的细胞色素氧化酶制剂。通过高效液相色谱法分离其亚基并测量每个多肽的放射性，能够确定复合物中细胞色素c对细胞色素氧化酶表面位点的屏蔽作用。
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