Four enzymes with phosphoglycolate phosphatase (EC 3.1.3.18) activity have been detected in extracts of chicken skeletal muscle and liver analyzed by gel-filtration and ion-exchange chromatography. 2. Two enzymes have been found in muscle extracts. One of them acts on glycerate 2,3-P2, in addition to glycolate 2-P. 3. Liver extracts contain two additional enzymes with broad specificity.